Vitamin A supplement during gonadal female maturation of astyanax altiparanae (characiformes: characidae)

Author(s)

Joseane Silva

• Document type: Master's Dissertation
• Press: Jaboticabal. 2019-09-12.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2019-06-26
• Advisor: Renata Guimarães Moreira; Daniel de Magalhães Araujo

Abstract

This study investigated the influence of vitamin A supplementation during the ovarian maturation of female lambari-do-rabo-amarelo, Astyanax altiparanae. 580 adult females were distributed in 10 tanks of 750 liters, composed by 5 treatments (duplicate). A basal diet with low level of vitamin A. The diet was supplemented with 0; 1.800; 3,600; 7,200 and 14,400 IU / kg of diet. After 8 days of adjustment to the experimental conditions with commercial diets, the fish received a diet without vitamin A supplementation for 16 days, for the depletion of these reserves and the animals were fed for 60 days with different treatments. Indicators of gonadal maturation, such as 17β-Estradiol (E2) plasma level, gonadosomatic index (GSI), fecundity and ovarian histology were analyzed at 30 and 60 days. Additionally, metabolic indicators such as hepatosomatic index (HSI), viscerosomal index (VSI) and plasma concentration of total lipids, triglycerides, total cholesterol and fractions were also analyzed at these periods. Vitamin A supplementation levels altered the deposition levels of this vitamin in the ovaries. The maintenance in the experimental conditions promoted ovarian maturation, evaluated by ovary histology and GSI. The physiological variables evidenced that the maintenance of these females in the experiment, with a diet composed entirely of vegetable ingredients, reduced the plasma levels of total lipids and cholesterol, and the higher level of supplementation (14,400 IU / kg) reduce also LDL levels. In addition, an intermediate concentration of supplementation (3,600 IU / kg) administered for 60 days increased the plasma levels of E2, but the mechanism that modulates this alteration has not been studied. Key words: Estradiol, lambari, lipid profile, vitamin A. (AU)

• FAPESP's process: 17/14699-7 - Vitamin A supplementation during the reproduction of Astyanax altiparanae females
• Grantee: Joseane da Silva
• Support Opportunities: Scholarships in Brazil - Master