Bioavailability and effects of L and DL-methionine supplementation on gut integrity and oxidative status of weaned pigs

Author(s)

Raphael Perini Caetano

• Document type: Master's Dissertation
• Press: Jaboticabal. 2019-12-06.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal
• Defense date: 2019-10-10
• Advisor: Luciano Hauschild; Paulo Henrique Reis Furtado Campos; Luan Sousa dos Santos

Abstract

Two studies were conducted to compare the relative bioavailability of L-Met and DL-Met based on N balance assay and the effects promoted by both sources on intestinal morphology and antioxidant system of weaned and growing pigs. In each experiment were used 42 barrows (Agroceres PIC, Rio Claro, SP, Brazil: CamboroughTM x AGPIC 337TM) with initial body weight of 10.7 ± 1.09 kg and 20.5 ± 1.55 kg, respectively for Experiment 1 and Experiment 2. Each experiment was divided into two batches with 21 pigs. Each batch corresponded to a block. Three pigs were randomly assigned to 7 experimental diets within each block, resulting in a total of 6 replicates per treatment. Basal diets (BD) were formulated with 68% methionine deficiency, but adequate for the other amino acids. Three graded levels of DL-Met and L-Met (0.03, 0.06 and 0.09%) were supplemented to the basal diet to create diets 2-7 in both studies. In experiment 1, both sources of methionine linearly reduced N excreted in the urine and linearly increased N retention (% absorbed) (P ≤ 0.03). The estimated relative bioavailability for L-Met compared to DL-Met was 106% (95% CI - confidence interval: 39 to 173%) for N retention (% absorbed) on an equi-molar basis. In experiment 2, supplementation with both Met sources linearly decreased urinary N excretion and linearly increased total N retention, N retention (% ingested and% absorbed) (P ≤ 0.04). The estimated bioavailability of L-Met compared to DL-Met was 95% (95% CI: 13 to 177%) for N retention (% of intake) and 94% (95% CI: 20 to 167%) for N retention (% absorbed N), respectively on an equi-molar basis. In both experiments, there were similar concentrations of carbonyl protein and MDA in plasma, duodenum and liver of pigs fed diets containing L-Met or DL-Met (P > 0.05). There were no statistical differences in villus height, villus width, crypt depth and villus height: crypt depth ratio of duodenum, jejunum and ileum for both Met sources (P ≥ 0.07). The results indicate that DL-Met and L-Met are equally bioavailable as methionine sources and positively impact on intestinal morphology and antioxidant system of weaned and growing pigs. (AU)

• FAPESP's process: 17/10354-5 - Bioavailability and effects of L-Methionine and DL-Methionine in the gut integrity and oxidative status of weaned pigs
• Grantee: Raphael Perini Caetano
• Support Opportunities: Scholarships in Brazil - Master