Association of chromogranin A and salivary amylase with anxiety and stress of hospital nursing professionals

Author(s)

Sérgio Valverde Marques dos Santos

• Document type: Doctoral Thesis
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Escola de Enfermagem de Ribeirão Preto (PCARP/BC)
• Defense date: 2019-09-09

Examining board members

Maria Lucia do Carmo Cruz Robazzi; Maria Helena Palucci Marziale; Luiz Almeida da Silva; Fábio de Souza Terra

• Advisor: Maria Lucia do Carmo Cruz Robazzi; Renata Roland Teixeira

Abstract

Nursing professionals working in hospital institutions are exposed to various stressful situations that can modify their physical and mental well-being, exposing them to stress and anxiety. Salivary Amylase (AA) and Chromogranin A (CgA) are promising biomarkers that can help in the diagnosis of these mental disorders, promoting a better prognosis for the worker. Objective: To analyze whether CgA and AA are associated with anxiety and stress in hospital nursing professionals. Method: This study was divided into two steps. In the first, a cross-sectional, descriptive-analytical, quantitative study was performed and, in the second, a longitudinal, correlational study. The research was conducted in a hospital institution in Southern Minas Gerais in 2018, with 210 nursing professionals. For data collection, a Characterization Questionnaire, the Beck Anxiety Inventory, was used to verify anxiety level and the Stress Symptom Inventory for LIPP Adults (ISSL) to measure stress. Saliva samples were collected from participants during work shifts. To verify AA activity, the Salivary Amylase Activity Determination method was used and the ELISA method was checked for CgA concentration. Data were tabulated and analyzed descriptively and inferentially using the Statistical Package for Social Science (SPSS) software and GraphPad Prism 5.0® software, using Pearson\'s chi-square, Fisher\'s exact test, Mann- Whitney and Logistic Regression, with a significance level of 5%. This study was approved by the Research Ethics Committee, according to Opinion Nº. 2.528.543. Results: Most professionals had stress (58%) and many of them (48,1%) had mild, moderate or severe anxiety. It was found that in the morning shift the activity of AA changed between the collection times. Already the CgA, presented more changes in the afternoon shift. It was found that the variables age group, number of children, length of work in the institution, weekly workload and medication use were significantly associated with the anxiety of nursing professionals (p<0,05). In addition, the variables: age group, physical activity practice, continuous medication use, length of work in the institution, other employment and smoking, showed an association with workers\' stress (p<0,05). Anxiety was found to be associated with stress of nursing professionals (p<0,001). When analyzing the association of anxiety and stress with AA and CgA, statistical significance was found between the group \"stress and anxiety\" with AA at night shift times (p<0,05). Significance was also found for the \"stress and anxiety\" group with CgA at night shift, as well as for the \"anxiety\" group at morning shift (p<0,05). Conclusion: Nursing professionals presented high levels of anxiety and stress, which were associated with changes in CgA and AA at certain times and work shifts, demonstrating that anxiety and stress biomarkers may be possible (AU)

• FAPESP's process: 16/02521-6 - Relationship of cromogranin a and salivar amylase with the anxiety and stress of hospital nursing professionals
• Grantee: Sérgio Valverde Marques dos Santos
• Support Opportunities: Scholarships in Brazil - Doctorate