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| Author(s): |
Dielson da Silva Vieira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Araçatuba. 2020-10-23. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba |
| Defense date: | 2020-10-14 |
| Advisor: | Francisco Leydson Formiga Feitosa; Pedro de Magalhães Padilha; Mateus Matiuzzi da Costa |
| Abstract | |
Goat's milk is a portion of nutritionally complex food and that by having several constituents with different bioactive potentials, they can be affected by some diseases, but when not, the consumption of milk and these compounds positively affect human and animal development. Thus, this study aimed to investigate the proteome and metabolome of goat's milk at different stages of lactation, age, and health standards (animals chronically positive for the caprine arthritis encephalitis virus-CAEV) as well as to evaluate in silico profiles of lactoferrin modeling from the milk of different mammals, contributing to studies on the milk of ruminants and other animals. To obtain the results, techniques such as 2D-PAGE, mass spectrometry, and in silico modeling were used with programs such as I-TASSER, Expasy ProtParam, COFACTOR, among others. In proteomic and metabolomic studies, animals were divided into groups, with at least seven and five animals per group, respectively. When the proteome of the transition from colostrum to the milk of goats was evaluated, a higher quality of proteins was observed in the animals that had their first calving, older animals in the production had the same proteins, but with lower quality. Likewise, with three days after delivery, the pattern of protein concentration has also changed, with, in general, less protein concentration compared to the day of birth. In the case of metabolites, younger animals had a higher number of metabolites than the older ones, and there was no difference in the metabolite profile between positive and negative animals for the CAE virus. Groups of metabolites have been identified as fatty acids (oleic acid, palmitic acid, etc.), sugars (sucrose, D-maltose, etc.), amino acids, and even other organic compounds (riboflavin). When the lactoferrin modeling was evaluated, this protein has its structure conserved in the genome of several mammal species, however, the structure of the goat protein has an addition of amino acids that do not affect the function or biological aspects of this protein. After model's generation, they were statistically validated according to the parameters established by the programs used, and the models generated from lactoferrin in the species Capra hircus and Macaca mulatta have the addition of some groups of amino acids, there is no change in the biological and chemical profile of these proteins when compared to other mammals. In this way, the data obtained provide information about the proteome and metabolome of goat's milk, as well as renew information about the modeling of proteins present in milk secretions, further contributing to the growth of data in dairy goat livestock and virus studies of goat encephalitis arthritis. (AU) | |
| FAPESP's process: | 17/22041-1 - Proteome from the transition from colostrum to milk in goats: protein interactions for the maturation of the immune and digestive systems of the newborn |
| Grantee: | Dielson da Silva Vieira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |