Analysis of nucleotide alterations in human papillomavirus 6 and 11 E6 region in Condyloma Acuminatum samples

Author(s)

Marina Carrara Dias

• Document type: Master's Dissertation
• Press: São José do Rio Preto. 2017-03-24.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto
• Defense date: 2017-03-06
• Advisor: Marilia de Freitas Calmon; Paula Rahal; Caroline Measso do Bonfim

Abstract

Condyloma acuminatum (CA) or genital warts are benign proliferative lesions that can be epidermal or mucous. Condyloma is caused by infection with human papillomavirus (HPV), mainly the low-risk types 6 and 11, but can also occur coinfection with high-risk HPVs. Human papillomaviruses have the capacity to infect the skin, oral and genital mucosa, and induce benign or malignant proliferative lesions. The variants of HPVs are defined as viral sequences that share identity in the nucleotide sequence of the L1 gene greater than 98%. Based on this criterion, HPV6 and 11 variants lineages have been studied and there is still an attempt to correlate these genetic variants with different clinical findings of infection. In this way, the aim of this study was to detect variants and nucleotide alterations presents in E6 region of HPV types 6 and 11 detected in condyloma acuminatum samples, to correlate the HPV presence with the clinical-pathological data of the patients and to determine phylogenetic relations of variants found with variants of others places of the world. The E6 region of 32 samples of condyloma were sequenced, being 25 positive to HPV6 and seven diagnosed HPV11. Twelve samples were identified as the HPV6a variant, and presented the mutation G474A, and one of these also showed the mutation T369G. The others 13 patients were positive to HPV6vc without nucleotide alterations. In the analysis of the seven HPV11 samples, it was observed that all patients showed the mutations T137C and C380T. Besides that, one patient also present the nucleotide alteration T410C. None of the mutations showed amino acid change. In the statistical analysis, it was observed a significant association between the HPV and HIV coinfection, and it was also found a significant association between HPV11 and alcohol consumption. Others data analyzed did not show significant association. In the phylogenetic analysis, it was observed that the samples were grouped according to variants, showing no relation to the geographical region that they were isolated. (AU)

• FAPESP's process: 15/06628-7 - Analysis of HPV11 variants in recurrent respiratory papillomatosis
• Grantee: Marina Carrara Dias
• Support Opportunities: Scholarships in Brazil - Master