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| Author(s): |
Hanyeny Raiely Leite Silva
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Jaboticabal. 2021-09-02. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2021-06-11 |
| Advisor: | Eduardo Festozo Vicente; Priscilla Ayleen Bustos Mac Lean |
| Abstract | |
The world's swine industry has been growing and solidifying pro-gressively in recent years. Thus, given the growing consumer demand for a higher quality and healthier product, the execution and application of new tech-nologies has become increasingly interesting. In this context, antimicrobial pep-tides (AMPs) represent an excellent alternative to replace commercial antimicro-bial additives. Therefore, the objective of this work is to synthesize and apply the antimicrobial peptide Ctx(Ile21)-Ha in formulations to act as an oral nutritional ad-ditive in piglets in the maternity phase, during the first 21 days of life. For this, the synthesis, purification and characterization of the antimicrobial peptide Ctx(Ile21)-Ha was performed, obtaining the molecule with high purity content and sufficient mass for in vivo studies. Then, the peptide was formulated via microencapsula-tion by ionotropic gelation and coated with hydroxypropylmethylcellulose phthalate (HPMCP), resistant to stomach pH. The microencapsulations were characterized by physicochemical methods and demonstrated that the peptide was adequately incorporated into the formulation. The evaluation of the peptide in vivo was divided into two experiments: the "Remediation", carried out in 10 animals as a preliminary evaluation, where the microparticles containing the pep-tide were applied in two stages to piglets already affected by diarrhea and the "Prophylactic /Therapeutic”, in which two different doses were applied in two treatments (PEP1 and PEP2, the latter receiving double the dose of the first), in addition to the control group (without pep-tide). All animals had access to ration in creep feeding until the day immediately before weaning (total = 136). The fecal score data for the Remediation and Prophylactic/Therapeutic experiments were submitted to correspondence analysis and the number of days to stop diarrhea and the number of days in each class of fecal score were calculated for each animal in both experiments . Both were analyzed using analysis of variance (ANOVA) and, regardless of the analysis, P-value ≤ 0.05 was considered signifi-cant. No significant effects were found in the Remediation experiments in any of the analyzed variables. However, in the Prophylactic and Therapeutic experi-ment, the average daily weight gain of the PEP1 treatment (0.214 kg/day) showed an increase of 18 g/day compared to the CRTL treatment (0.196 g/day) and 9 g/day a more in relation to the PEP2 treatment (0.205 kg/day) during the 21 days of the maternity phase (Pvalue < 0.0001). Weaning weight in the PEP1 treatment (6.01 kg) (lower dose of peptide) was significant (Pvalue ≤ 0.05), indicating a final mean increase of 0.30 g per animal in relation to the CRTL treatment (5.71 kg) and a mass value of 0.19 g higher in relation to the PEP2 treatment (5.82 kg). However, for the presence of diarrhea, fecal score and the number of days for diarrhea onset and cessation, for both experiments, there was no significant dif-ference (Pvalue > 0.05), also observed for the experiment's GPD variable of Re-mediation. In conclusion, the antimicrobial peptide Ctx(Ile21)-Ha can be consid-ered an interesting substitute for commercial antibiotics for application in swine, as it presented a similar performance as the commercial growth promoters used. (AU) | |
| FAPESP's process: | 18/25735-7 - Synthesis and application of the antimicrobial peptide Ctx(Ile21)-Ha and its conjugates in different formulations as a nutritional additive in swines |
| Grantee: | Hanyeny Raiely Leite Silva |
| Support Opportunities: | Scholarships in Brazil - Master |