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Use of freeze-dried cat sperm fot the in vitro production of embryos by usung intracytoplasmic sperm injetion (ICSI)

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Luis Carlos Oña Magalhães
Total Authors: 1
Document type: Doctoral Thesis
Press: Botucatu. 2015-03-03.
Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
Defense date:
Advisor: Maria Denise Lopes

The in vitro production of embryos (IVP) has been a good alternative for conservational programmes of wild felids threatened by extinction. However, IVP depends upon several other assisted reproduction techniques such as in vitro maturation (IVM), in vitro fertilization (IVF), embryo transfer (ET), gamete cryopreservation. These techniques were already successfully employed in wild felines. Freeze-dried sperm has also been used to produce live offspring of several species, but in domestic cat this has not been reported yet. Hence, the objective of the present study was to verify the sperm DNA fragmentation after the freeze-drying process as well as the feasibility of using freeze-dried sperm together with ICSI to produce cat IVP embryos. Fresh, frozen/thawed, freeze-dried sperm samples of 3 normospermic cats were used in this experiment. Sperm DNA integrity assessments were performed (Conventional Acridine Orange, Alkaline Comet Assay, Acridine Orange using flow cytometry, and TUNEL). TUNEL assessment showed higher sensitivity to detect DNA fragmentation and it detected higher DNA fragmentation in freeze-dried sperm when compared to frozen/thawes and fresh semen from the same donors. It was not possible to produce IVP embryos making use of freezedried sperm that used SOF as medium and ICSI (AU)

FAPESP's process: 11/00072-6 - The use of domestic cat (Felis silvestris catus) freeze-dried sperm to produce in vitro embryos via intracytoplasmic sperm injection (ICSI)
Grantee:Luis Carlos Oña Magalhães
Support Opportunities: Scholarships in Brazil - Doctorate