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| Author(s): |
Leonardo Lucas Carnevalli Dias
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2009-04-16 |
| Examining board members: |
Eny Iochevet Segal Floh;
Claudete Santa Catarina;
Miguel Pedro Guerra;
Fanly Fungyi Chow Ho;
Carlos Alberto Labate
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| Advisor: | Eny Iochevet Segal Floh; Claudete Santa Catarina |
| Abstract | |
The great similarity among the events of zygotic and somatic embryogenesis allows the establishment of parameters for accompaniment of these processes. In the present work it was studied the somatic embryogenesis in Ocotea catharinensis, in the maturation and germination phases, and the zygotic embryogenesis. The biochemical parameters and differential expression of proteins were evaluated: The treatment with ABA + PEG presented similar variations for zygotic embryos in the maturation stage. The germination was not observed for somatic embryos; however, it was verified that the previous dehydration promoted important biochemical alterations. With relation to the zygotic embryo, throughout the germination process, the synthesis of new proteins was not observed. The proteomic studies carried out throughout seed development, allowed the selection of polypeptides with differential expression. The results obtained open perspectives for the methodology optimization of the somatic embryogenesis, for species with recalcitrant seeds, like O. catharinensis. (AU) | |
| FAPESP's process: | 05/58208-0 - Physiological, biochemical aspects and comparative proteomic analysis during the maturation, germination and conversion into plants of embryos of Ocotea catharinensis Mez. (Lauraceae) |
| Grantee: | Leonardo Lucas Carnevalli Dias |
| Support Opportunities: | Scholarships in Brazil - Doctorate |