Molecular phylogeny of Tachinidae (Diptera, Brachycera, Calyptratae) based on next-generation sequencing, focusing on subfamilies boundaries and relationships

Author(s)

Letícia Chiara Baldassio de Paula

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB)
• Defense date: 2021-04-28

Examining board members

Silvio Shigueo Nihei; Kirstern Lica Follmann Haseyama; Daniela Maeda Takiya

• Advisor: Silvio Shigueo Nihei; Daniel José Galafasse Lahr

Abstract

The Tachinidae is one of the largest families of Diptera. They are found in nearly all terrestrial environments throughout the world, including deserts, forests, grasslands, mountains, and tundra. The first phylogenomic analyses of Tachinidae using transcriptomic data is presented here. The analyses are based on 36 species for the ingroup, composing the tachinids, and 23 species for the outgroup, representing the other Oestroidea families. Were constructed three datasets: 100% coverage (92 single-copy protein-coding genes), 75% coverage (1304 single-copy protein-coding genes), and 50% coverage (1890 single-copy protein-coding genes). Overall, the obtained topologies are well resolved, with strong node support and small changes among the trees obtained with variation of coverage and different approaches (maximum likelihood, maximum parsimony). Most nodes in Maximum Likelihood analyses have 100% ultrafast bootstrap support. The Maximum Parsimony analyses also show stability with 100% support with jackknife and bootstrap in almost every node. However, the maximum parsimony tree presents more variation with Bremer support ranging from 125 to 18442. The analyses recovered the four tachinid subfamilies with the following relationship: Phasiinae + Dexiinae and Tachininae + Exoristinae. Interestingly, the tribe Myiophasiini (Tachininae) forms a different lineage, a clade sister to all the remaining Tachinidae. The Neotropical tribe Iceliini (former Tachininae) is recovered within Exoristinae, sister to Winthemia. Also, Masyphyini (former Exoristinae) is recovered within Dexiinae, close to Dexiini. In general, results presented herein are congruent with recent studies that include tachinids. Hopefully, these results can be a scaffold for future studies with Tachinidae phylogeny. (AU)

• FAPESP's process: 19/00366-1 - Molecular phylogeny of Tachinidae family (Diptera, Brachycera, Calyptratae) based on next generation sequency, focusing on subfamily relationships and limits
• Grantee: Letícia Chiara Baldassio de Paula
• Support Opportunities: Scholarships in Brazil - Master