Full text
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| Author(s): |
Bianca Maira Teixeira Ayres
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química |
| Defense date: | 2010-07-26 |
| Examining board members: |
Telma Teixeira Franco;
Maria Isabel Felisberti;
Martin Georg Peter
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| Advisor: | Gustavo Paim Valença; Telma Teixeira Franco |
| Abstract | |
The optimal conditions for the enzymatic production of fructose acrylates were studied. Fructose to acrylic acid molar ratio, the amount of immobilized lipase Candida antarctica and the influence of molecular sieve in the reaction media were studied. The results of these variables in converting sugar were monitored. Enzymatic esterifications are equilibrium reactions whose subproduct is water. The presence of this must be controlled to allow enzymatic activity of the lipase and to avoid hydrolysis of esters. The addition of molecular sieve 3 A to the system was investigated to remove of produced water and to shift the equilibrium to the synthesis of ester. The exclusive production of monoester was observed when the molar ratio of fructose: acrylic acid of 1:3, 55 °C, 200 rpm and 20 mg ml-1 of immobilized lipase was employed. When the molar ratio is increased the conversion of fructose increases, but the equilibrium was shifted to the production of di- and triester. The addition of 3 g of molecular sieve resulted in the production of 49.5 mM of fructose monoacrylates, 84 % conversion of the initial fructose and 41 % conversion of acrylic acid after 24 hours of reaction. Since sugar acrylates are not available, it were obtained the concentrations of esters from the determination of response factors of each ester by HPLC-RI based on linear regression (AU) | |
| FAPESP's process: | 08/02235-7 - Production of frutose acrylates by biocatalysis |
| Grantee: | Bianca Maíra Teixeira Ayres |
| Support Opportunities: | Scholarships in Brazil - Master |