Synthesis, characterization and processing of bioreabsorbable polymers to use in Tissue Engineering

Author(s)

Ana Flávia Pattaro

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia Química
• Defense date: 2016-07-06

Examining board members

Rubens Maciel Filho; Cecília Amélia de Carvalho Zavaglia; Rodrigo Alvarenga Rezende; Adriano Pinto Mariano; Fernando dos Santos

• Advisor: Rubens Maciel Filho; Maria Ingrid Rocha Barbosa Schiavon

Abstract

The search for materials that can replace total or partial functions of an organ or tissue have gained attention and commitment of the scientific world in recent years. Obtaining biomaterials with the necessary characteristics for medical applications and which show no toxicity is critical to the success of Tissue Engineering. Thus, this project has been prepared seeking the synthesis, characterization and processing of biomaterials for use in Tissue Engineering. The study involves the process of in situ polymerization of Poly (L-Lactide) (PLLA) and Poly (Glycolide) (PGA) and their copolymers PLGA. It was also obtained organoclay for the production of nanocomposites PLLA using the process of in situ polymerization. The kinetics of ring opening polymerization of PLLA was performed by using DSC technique. Comparisons of Activation Energy (Ea) were made by using three isoconversinal methods: Ozawa ¿ Flynn ¿ Wall (OFW), Kissinger ¿ Akahira ¿ Sunose (KAS) and Vyazovkin. The PLLA and PGA polymers produced different amounts of catalyst, initiator and time reaction were characterized by FTIR, TGA, DSC, GPC and XRD techniques. The PLGA copolymers have also been characterized by FTIR, DSC and TGA. The organophilization process performed for bentonite clay occurred in a satisfactory manner and was used to obtain the nanocomposite. A new process has been developed for obtaining nanocomposite PLLA, which was the ring opening polymerization without blanketing the reaction medium and the addition of clay was slowly after complete melting of the monomer. The result of this new process produced a nanocomposite of PLLA with evidences that was completely exfoliated. The processing of the nanocomposite PLLA and pure PLLA was performed using the electrospinning techinique. Morphological and biological analyzes of the resulting nanofibers were made and the tests performed indicate that the pure and nanocomposite PLLA materials does not present toxicity to the medium of studied culture (AU)

• FAPESP's process: 12/06545-6 - Synthesis, Characterization and Processing of Bioabsorbable Polymers to Use in Tissue Engineering
• Grantee: Ana Flávia Pattaro
• Support Opportunities: Scholarships in Brazil - Doctorate