Study of action of hyperbaric oxygenation in Pseudomonas aeruginosa.

Author(s)

Flávia Luna Lima

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2011-06-21

Examining board members

Selma Giorgio; Domingos da Silva Leite; Rodrigo Ramos Catharino

• Advisor: Luciana Maria de Hollanda; Selma Giorgio

Abstract

Pseudomonas aeruginosa is a major opportunistic pathogens responsible for nosocomial infections. These infections represent a serious health problem for people with severe wounds and burns and are responsible for high mortality rates in these patients. The chronically injured tissues have small areas of hypoxia, these areas, low vascularization, are extremely difficult to treat, reduce and alter the effects of medicines providing that multiresistant strains of this pathogen to infect and survive in these places. Thus, it is essential to develop a treatment that is effective both in combating the pathogen, as in the regeneration of damaged tissue. Therapy with hyperbaric oxygen (HBO) promotes tissue regeneration by new vessel formation, has bacteriostatic and bactericidal properties and can act synergistically with antibiotics. The aim of this study was to evaluate the role of HBO on the growth of P. aeruginosa, in the imipenem action and infected macrophages and artificial skin infected with this pathogen. Our results show that exposure of six strains of P. aeruginosa to hyperbaric conditions (100% O2 at 3 ATA pressure) reduces bacterial growth, and maximize the effect of imipenem/ cilastatina against this bacterium. Exposure to HBO for 90 minutes protected macrophages from lysis caused by bacterial infection. Artificial skins infected with P. aeruginosa and exposed to HBO showed a decrease in bacterial load. These results support the importance of hyperbaric oxygen therapy in control infections caused by this pathogen (AU)

• FAPESP's process: 09/01817-5 - Study of the effectiveness of hyperbaric chamber (HBO) in the treatment of Pseudomonas aeruginosa in vitro and in murine models
• Grantee: Flávia Luna Lima
• Support Opportunities: Scholarships in Brazil - Master