Full text
| |
| Author(s): |
Mariana Silveira Derami
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2019-11-14 |
| Examining board members: |
Wanderley Dias da Silveira;
Domingos da Silva Leite;
Márcio André Miranda
|
| Advisor: | Wanderley Dias da Silveira; Renato Pariz Maluta |
| Abstract | |
The presence of diseases in the lungs alters the conditions necessary for microbial growth in the respiratory tract and may change the composition of the resident microbiota. In birds, Avian Pathogenic Escherichia coli (APEC) strains are associated with diseases of the respiratory tract. Little is known about the microbiota of the respiratory tract of birds. In this study, we identified and compared the respiratory tract microbiome of healthy chickens (n = 6) and chickens diagnosed with colibacillosis (n = 14) by collecting lung and tracheal fragments. The microbiome was accessed using the amplification of 16S rRNA gene and next-generation sequencing (NGS) technique. No significant difference was observed when comparing the average number of taxa detected in the healthy chickens (153.5 ± 64.22) to the chickens diagnosed with colibacillosis (101.57 ± 58.43) (p = 0, 0935), as well as in the evaluation of richness and diversity by Chao1 and Shannon indices. The most abundant taxa found in samples from diseased chickens were Eschericha / Shigela and Lactobacillus with an abundance of 36.6 and 50.3 times compared to healthy chickens, respectively. In healthy chickens¿ samples the most common taxon was Staphylococcus. The results show that there is a tendency to decrease microbial richness and diversity in the respiratory tract of chickens diagnosed with colibacillosis. Through the pulsed field electrophoresis (PFGE) technique, the genomic profile of 22 positive E. coli isolates for all APEC markers (ompT, iss, hlyF, iroN and iutA) were distributed in 14 pulsotypes. The sequencing of one isolate from each pulsotype allowed the assembly of a phylogenetic tree. Comparison between the phylogenetic tree to the dendrogram presented a different pattern in the assembling of the samples (AU) | |
| FAPESP's process: | 17/21904-6 - Comparison between APEC genomes and genomes of commensal Escherichia coli |
| Grantee: | Mariana Silveira Derami |
| Support Opportunities: | Scholarships in Brazil - Master |