Desenvolvimento e caracterização de carreadores lipídicos nanoestruturados para incorporação de beta-caroteno

Author(s)

Fernanda Luisa Lüdtke

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos
• Defense date: 2021-12-16

Examining board members

Ana Paula Badan Ribeiro; Ana Silvia Prata; Guilherme José Maximo; Severino Matias de Alencar

• Advisor: Ana Paula Badan Ribeiro; António Augusto Vicente

Abstract

Nanostructured lipid carriers (NLC) were developed as a system for entrapment and delivery of liposoluble bioactive compounds, which due to their chemical instability and low water solubility, have low bioavailability. Composed of lipids that have high and low melting points, CLN exhibit several potential advantages that can be exploited through the choice of materials used in their composition. Thus, the objective of this work was to obtain ?-carotene loaded NLC, through lipid matrices commonly used in the oil and fat industry. For this purpose, the work was divided into four stages. The first step consisted of optimizing the process conditions of high pressure homogenization (HPH) and evaluating the performance of synthetic and natural emulsifiers to obtain NLC. Enzymatically modified soy lecithin (SL), ethoxylated sorbitan monooleate (Tween 80) and whey protein isolate (WPI), the pressure of 700 bar and 2 homogenization cycles were defined as effective and optmized process conditions and components for obtaining NLC in subsequent stages of the work. The second stage was related to the obtainment, characterization, and stability study of NLC from different lipid systems composed of fully hydrogenated soybean oil (FHSO) and high oleic sunflower oil (HOSO. The lipid systems were characterized in macroscale in terms of composition, physical and crystallization properties, and from this, FHSO: HOSO (w/w) 80:20, 60:40, 40:60 and 20:80 lipid systems were defined to obtain NLC. Among the lipid systems considered, the 60:40 FHSO: HOSO (w/w) system provided the obtainment of stable particles with adequate crystallinity characteristics for entrapment of the lipophilic bioactive compound of interest. So, in the third step, the potential of NLC for entrapment of lipophilic bioactive compounds was evaluated. In this sense, ?-carotene loaded NLC obtained with three different emulsifiers (SL, Tween 80 and WPI), were evaluated for the encapsulation efficiency and loading capacity, as well as the evaluation of physical stability, crystalline and polymorphic stability. NLC obtained using SL and Tween 80, showed greater potential for of ?-carotene entrapment, being thus selected for the study of in vitro digestibility in the subsequent step. In the fourth step studies related to behavior of NLC during in vitro dynamic digestibility, ?-carotene bioaccessibility and cytotoxicity of NLC and the components used in its composition were carried out. The NLC obtained with Tween 80 showed greater stability during the passage through the TGI and provided greater protection and delivery of ?-carotene. The cell viability found after cytotoxicity assays indicated that both NLC do not present toxic effect at the tested concentrations (1- 25 ?g.ml-1). The use of FHSO and HOSO as lipid matrices provided the obtainment of NLC with high crystal stability, good load capacity and protection of the incorporated bioactive compound, which are biocompatible and presents a viable cost for application in foods (AU)

• FAPESP's process: 18/03172-0 - Development of nanostructured lipid carriers for incorporation of lipophilic bioactive compounds
• Grantee: Fernanda Luisa Lüdtke
• Support Opportunities: Scholarships in Brazil - Doctorate