Arrabidaea chica Verlot: controled release formulations with aplications for ulcer lesions

Author(s)

Leila Servat Medina

• Document type: Doctoral Thesis
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2014-02-28

Examining board members

Mary Ann Foglio; Angela Maria Carneiro Araújo; Maria Helena Sarragiotto; Fernando Antonio Cabral; Patricia Correa Dias

• Advisor: Rodney Alexandre Ferreira Rodrigues; João Ernesto de Carvalho; Mary Ann Foglio

Abstract

Arrabidaea chica, known as crajiru, is part of the national Unified Health System from Brazil (SUS) list of medicinal plants of interest. Studies conducted at CPQBA- UNICAMP showed that the species demonstrated wound healing, antiulcerogenic and antioxidant properties. This study aimed the evaluation of pharmaceutical systems employing standardized extract for treatment of skin and mucosal wounds. Chitosan nanoparticles were obtained using the crosslink method. The protocol that showed best conditions to produce nanoparticles, among those tested, used tripolyphosphate as a cross-linker, in 1:5 (mass) and 1:10 (volume) proportions compared to chitosan. For topical applications, A. chica nanoparticles were incorporated into sponges, polyvinyl alcohol absorbable films or hyaluronic acid hydrogels. In vitro studies with human fibroblasts showed the activity of A. chica nanoparticles in cell proliferation and migration. In vivo studies with rats and hamsters provided the standardization parameters for gastrointestinal and mucositis models respectively. A chica nanoparticles demonstrated inhibition of mucosal ulcerations of gastric ulcer induced by ethanol or indomethacin models, improving ulcerative wound healing by 76% and by 58% respectively, in comparison to negative control groups. Those same samples incorporated into absorbable films or hydrogels were evaluated on wound healing activity using a rat dermal ulcer model. Animals treated with loaded films with 0.5 mg of nanoparticles achieved 79% in wound area contraction, whereas animals receiving hydrogels containing 1.5 mg of nanoparticles achieved 85%. These results showed that the production of A. chica nanoparticles and transport systems were practicable, demonstrating to be a valid alternative for incorporation of A. chica extracts, allowing decrease of the required dose to achieve the desired effect (AU)

• FAPESP's process: 09/16484-1 - Arrabidaea chica Verlot: Controled release formulations with aplications for ulcer lesions.
• Grantee: Leila Servat Medina
• Support Opportunities: Scholarships in Brazil - Doctorate