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| Author(s): |
Bruno Dias Nani
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2016-02-23 |
| Examining board members: |
Michelle Franz Montan;
Daiane Cristina Peruzzo;
Erika Harth Chu
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| Advisor: | Fernanda Klein Marcondes; Michelle Franz Montan; Karina Cogo Müller |
| Abstract | |
Recently it was demonstrated that academic stress is able to increase emanation of oral Volatile Sulphur Compounds (VSC), the gases that originate halitosis. These subjects also showed an increased salivary concentration of Alpha-amylase (AA) and Mucin 5b (MUC) and a decreased concentration of Beta-defensin (BD). These findings indicate that altered saliva represents a favorable environment for bacterial VSC production in oral cavity, but this has not been confirmed yet. The aims of this study were to evaluate the relationship between salivary bacterial amount, oral emanation of VSC and academic stress in healthy subjects, and if AA, BD and MUC are able to alter bacterial viability in vitro. In a previous study, 78 men students were evaluated for the presence of stress by quantifying the burnout syndrome and oral emanation of the VSC H2S, CH3SH and (CH3)2S by Oral Chroma¿. In the present study, these salivary samples were grouped in either "Stress" ("S") or "No Stress" ("NS") groups. Total bacterial counts and seven halitosis-related bacteria were quantified by qPCR. Data were compared between groups and correlated with VSC emanation by previous study subjects. Two bacteria were selected for an in vitro evaluation of its growth in presence of alpha-amylase (AA), beta-defensin (BD) and mucin (MUC). According to burnout syndrome quantification, 21 subjects were classified in "Stress" group and 57 in "No Stress" group. "S" group showed increased amounts of H2S, (CH3)2S and Solobacterium moorei (Sm) (p < 0.05, Mann Whitney). There was a moderate positive correlation between Fusobacterium nucleatum (Fn) and H2S, Fn and CH3SH; Sm and CH3SH; Sm and Fn; Tannerella forsythia (Tf) and Sm; Tf and Fn (p < 0.05, Spearman correlation). AA, BD and MUC did not alter the in vitro growth of Sm and Fn (p> 0.05, one way ANOVA). These results indicate that increased oral emanation of H2S was related to the increase in Sm salivary amount and its interaction with Fn and Tf, indicating that these bacteria are important for the development of halitosis due to emotional disorders. It remains unknown the mechanisms responsible for the increase in oral emanation (CH3)2S (AU) | |
| FAPESP's process: | 13/26691-0 - Relationship between stress and oral microbiota: the effect on the production of volatile sulphur compounds and the role of beta-defensin |
| Grantee: | Bruno Dias Nani |
| Support Opportunities: | Scholarships in Brazil - Master |