Study of the bone response of an implant surface coated with odanacatib through the analysis of wettability, topography, computed microtomography, histological and histometric

Author(s)

Natália dos Santos Sanches

• Document type: Master's Dissertation
• Press: Araçatuba. 2023-03-01.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
• Defense date: 2023-02-17
• Advisor: Idelmo Rangel García Júnior

Abstract

Currently, new active principles of anti-absorptive function have gained the field of study to evaluate their mechanisms and biological behavior. Thus, one of the most significant challenges is to impregnate these principles on the surface of the titanium implant and stimulate new bone formation and decrease the long-term reoperation action. The objective was to evaluate the dynamics of the bone response of a surface modified by the double acid attack (GC), double acid attack and body fluid solution (GX), and the active ingredient Odanacatib (GO) at a concentration of 0,26mg/ml using the biomimetic method in a standardized model in rat tibia. 48 male rats (Wistar), 24 discs, and 102 titanium implants were designated and subdivided between the groups for the analysis of wettability, topography, computed microtomography, histological, and histometric. In scanning electron microscopy all groups showed a homogeneously rough and irregular surface, with hydroxyapatite deposition in GX and GO. In the energy dispersive X-ray spectroscopy the GC presented pure Ti, the GX presented Ti, O, Na, P, Ca, and Cl, and the GO Ti, O, Si, P, Cl, and Ca. In the contact angle analysis, the GX and GO presented a hydrophilic surface for both tested solutions, distilled water and GC showed a hydrophilic profile in the distilled water solution and the hydrophobic SFC. In the histological analysis, it was possible to observe a similar behavior between the groups in the bone neoformation and corticalization in the bone/implant interface area. For the evaluation of quantitative data was performed ANOVA 2 factors, with the post-Tukey test adopting the significance level (p <0.05). Wettability was statistically different between GC and GO for the distilled water solution (p=0.0007), and for SFC between GC and GX (p=0.0145) and GC and GO (p=0.0007). In the microtomographic parameters, a statistically significant difference was obtained for Tb.sp at 15 days between the GC and GX groups (p=0.0043) and at 40 days between the GC and GO groups (p=0.0314), and for Conn.Dn. at 15 days between the GC groups with GO (p=0.0008) and X with GO (p<0,001)at 40 days for CG with GX (p=0.236) and GX with GO (p=0.227), and in intragroup comparison between GO 15 days with GO 40 days (p=0.0227). In the histometric for NBA, there was a significant statistical difference in the intergroup comparison in the period of 15 days for GC with GO (p=0.035) and for GX with GO (p=0.0347), and intragroup between the periods of 15 days and 40 days for GC (p=0.0001), for GX (p=0.0001) (p<0.0001). For the values of ELCOI obtained significant statistical differences in the intragroup comparison between the periods of 15 and 40 days for the GC (p=0.0089) and for the GO (p = 0.005). The surface modifications were effective for the osseointegration of implants in normophysiologic organisms, evidencing the topical effect of deposition of Odanacatib by the biomimetic method when presenting a substantial increase in bone formation and surface corticalization from 15 to 40 days. (AU)

• FAPESP's process: 21/08067-3 - Study of bone response of implant surface coated with odanacatib through wettability, topographic, cell culture, histomorphometry and computed microtomography analyses
• Grantee: Natália dos Santos Sanches
• Support Opportunities: Scholarships in Brazil - Master