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| Author(s): |
Marina Rodrigues de Abreu
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Rio Claro. 2023-03-01. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro |
| Defense date: | 2023-01-06 |
| Advisor: | Maria Izabel Souza Camargo |
| Abstract | |
The species Rhipicephalus sanguineus l. s. (R. linnaei), better known as the “dog tick”, is of great medical and veterinary importance, not only for despoiling its hosts, but also for being a potential vector of pathogenic agents. In this way, numerous studies have been searching effective methods to control this hematophagous, and currently, the most used strategies for its control have been the use of synthetic chemical acaricides, however, the indiscriminate application of these chemicals has caused enormous disadvantages such as the selection of more resistant ticks, risk of toxicity to hosts and human health, and environmental contamination. Therefore, new effective alternatives to control this ectoparasite need to be evaluated. Ozone has been reported in the literature as an oxidizing agent with potential antimicrobial, disinfectant, sterilizing and healing action and its properties have been widely explored in the food industry, in the disinfection of hospital environments, and recently also in the control of agricultural pests. In this sense, the hypothesis of its action as an acaricidal agent for the control of ticks arose, and although some information in the literature already indicates this path, little has been studied regarding its acaricidal effect and mainly how the mechanisms of action on the exposure of ticks work in tissues and cells of these ectoparasites to this substance. Given the above, the present study proposed to study the mechanisms of action of ozone (ozonized water) on adult females of the tick R. sanguineus l. s. (R. linnaei) in vivo and embryonic lineage cell cultures (RML-RSE and ISE6) in vitro evaluating the effects presented by this substance in different cellular and tissue models. The results showed that ozone (water and ozonized oil) can be considered a potential natural acaricide with action on ticks of the species R. sanguineus l.s. (R. linnaei), causing toxicity in these, confirmed by the morphophysiological changes found both in the organs of these ectoparasites (salivary glands, ovary, organ of Gené and integument), as well as altering reproductive biological parameters, cell viability and gene expression of embryonic lineage tick cells. (AU) | |
| FAPESP's process: | 18/02999-9 - In vitro and in vivo action of ozone on embryonic lineage cells (RML RSE and RML-15) and R. sanguineus s. l. semi engorged females |
| Grantee: | Marina Rodrigues de Abreu |
| Support Opportunities: | Scholarships in Brazil - Doctorate |