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| Author(s): |
Ludimila Dias Almeida
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2022-01-28 |
| Examining board members: |
Elizabeth Bilsland;
Andrea Balan;
Nádia Maria Vieira Sampaio;
Carolina Borsoi Moraes;
Mário Henrique Bengtson
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| Advisor: | Elizabeth Bilsland |
| Abstract | |
The yeast Saccharomyces cerevisiae is an important research model organism. It is an extensively studied eukaryote, for which there is a considerable number of genes orthologous to human genes. Different molecular biology strategies have been developed and applied to yeast. We can highlight the fact that it is the first organism with complete libraries of non-essential and essential gene deletion available to the scientific community. In addition, techniques for large-scale construction of new libraries of interest, such as the Synthetic Genetic Array (SGA), are available. Using these technologies, strategies for the study of new molecules in the scope of discovering new drugs have been employed in this organism. Approaches such as the chemical genomic profile, which uses the deletion libraries, allow us to understand and identify genes and metabolic pathways participating in the target processes of a molecule of interest. This organism can also be used as a platform for the expression of genes involved in diseases, not only to understand the role of this gene in cell metabolism, but also to search for molecules that change the phenotype caused by it in the cell. Considering the power of yeast as a platform for the discovery of new drugs, this work presents different approaches that exemplify the applicability of this organism in this area of great interest. In Chapter I we present the use of a yeast library of double mutants for genes encoding non-essential transporters, in small- and large-scale screenings, for identifying transport routes for commercial compounds of medical and agricultural interest. In Chapter II we present two strategies in yeast to search for compounds that act in the alteration of the aggregation and cytotoxicity phenotypes caused by expression of proteins involved in Amyotrophic Lateral Sclerosis (ALS). In summary, in this work we explore the versatility of the yeast Saccharomyces cerevisiae to answer important questions in the process of developing new drugs (AU) | |
| FAPESP's process: | 17/01986-8 - Determination of substrate specificity of plasma membrane transporters from Saccharomyces cerevisiae and Homo sapiens |
| Grantee: | Ludimila Dias Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate |