Determination of aflatoxin M1 and cyclopiazonic acid in fluid milk commercialized in São Paulo-SP, Brazil

Author(s)

Josué Fontes Rosmaninho

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Saúde Pública (FSP/CIR)
• Defense date: 2004-04-05

Examining board members

José Cezar Panetta; Benedito Correa; Evelise Oliveira Telles

• Advisor: José Cezar Panetta

Abstract

The co-occurrence of aflatoxin Mt (AFM1) and cyclopiazonic acid (CP A) was surveyed in 40 samples of fluid milk commercialized in São Paulo and complementary was estimated the level of population exposition, by using estimated of daily intake of toxins from analytical results of positives samples and by mean consumption of varied types of milk. To this end, were collected samples of pasteurized milk types A, B, C and ultra-high temperature, from two different brands, in markets and bakeries in São Paulo. Each milk sample was constituted by one original pack of 1 L, representative of one production batch. Samples were collected during January to May 2002. The AFM1 analysis were done by high performance liquid cromatography (HPLC), using immunoaffinity columns to purification. The CPA analysis were done by extraction with polar solvents, followed by clean-up in a silica gel column and quantitative determination by thin layer chromatography (TLC). The analytical methods used to determine AFM1 e CPA were evaluated experimentally through repeated analysis of milk samples spiked with both toxins. Results showed 23 (57,5%) positive samples for AFM1 at levels of 10,6- 121,2 ng/L of milk. The mean levels of AFM1 in milk type A, B, C and ultrahigh temperature were 5,9; 26,6; 19,8 e 22,2 ng/L, respectively. CPA was not detected in milk samples; the detection limit was 3 μg CPA/L of milk. By using data on milk consumption of 720 ml for children aging 4 months and using mean weight of 7 Kg, was estimated the mean daily intake of 0,18; 1,64; 1,22 e 1,82 ng of AFM1/ Kg body weight/ day, for milk types A, B, C and ultra-high temperature, respectively. Are discussed the implications of these data on Public Hea1th and the approaches for the establishment of regulations for AFM1 in milk and milk products. (AU)