Full text
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| Author(s): |
Isis Fiéri
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2022-08-26 |
| Examining board members: |
Jackson Cioni Bittencourt;
Gabriela Placoná Diniz;
Ana Carolina Takakura Moreira;
Alexandre Leite Rodrigues de Oliveira
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| Advisor: | Jackson Cioni Bittencourt |
| Abstract | |
Cerebrospinal fluid (CSF) is the fluid that makes up the majority of the extracellular fluid of the central nervous system (CNS). It is important in mechanical protection, osmotic buffering, and chemical homeostasis of the CNS, as well as communication and neuroendocrine integration. The choroid plexus (PC), a highly vascularized secretory epithelium that is the primary site of augurin production, produces it. Augurin is a peptide encoded by the esophageal cancer-4 (Ecrg4) gene, also known as C2orf40 in humans. This peptide functions as a tumor suppressor gene, inducer of neural senescence, immune system regulator, and potentiator of hypothalamic adrenocorticotropic hormone (ACTH) release. It is widely distributed throughout the body\'s organs, with a focus on those related to the CNS and cardiovascular system (CVS). The brain-heart axis connects the two systems, which are critical for maintaining body homeostasis. This axis is made up of a network of limbic and autonomic structures that are linked to theta rhythm. Because augurin is a relatively new and understudied peptide, the goal was to perform neuroanatomical mapping, quantification in CSF and serum, and gene expression verification (RT-qPCR) in 1-month, 3-month, and 12-month rats. The findings revealed intense immunostaining for augurine in limbic areas of the brainstem-hippocampus ascending synchronization pathway, significantly higher levels of augurin in cerebrospinal fluid compared to blood, and Ecrg4 gene expression in all brain areas analyzed, with differences related to age and sex. Augurin is thus a peptide hormone with a significant role in the integration and communication of the brain-heart axis and, as a consequence, in homeostatic regulation. (AU) | |
| FAPESP's process: | 19/01820-8 - Neuroanatomic characterization and dimorphic aspects of ECRG4/augurin in rats (Rattus norvegicus - long-evans) during the aging process |
| Grantee: | Isis Fieri |
| Support Opportunities: | Scholarships in Brazil - Master |