Full text
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| Author(s): |
Gabriel Calheiros Antunes
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Limeira, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas |
| Defense date: | 2024-08-30 |
| Examining board members: |
José Rodrigo Pauli;
Alisson Luiz da Rocha;
Vitor Rosetto Muñoz
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| Advisor: | José Rodrigo Pauli |
| Abstract | |
Erratic eating patterns, such as those experienced by shift workers, can worsen obesity and metabolic steatotic liver disease (MSLD). Time-restricted eating (TRE) has emerged as a potential strategy to mitigate the effects of obesity, supported by several studies on its benefits for metabolic disorders like MSLD. Regular physical exercise is recommended as a non-pharmacological method to address obesity and related issues. Both TRE and exercise show promising independent improvements in metabolic health, weight, and glycemic control. Furthermore, pharmacological strategies involving circadian cycle proteins, such as Rev-erb?, are potential therapies against obesity and MSLD. Thus, combining both approaches could be a promising strategy to combat obesity and MSLD. Male and female C57BL/6J mice, six weeks old, were used and subjected to an obesity induction protocol for eight weeks, followed by isolated TRE, TRE combined with aerobic exercise, and TRE combined with the administration of SR9009 in obese mice. It was observed that the treatment of obesity and MSLD through the TRE strategy, even during the animals' inactive window, showed positive effects on weight loss, adiposity, glycemic homeostasis, insulin sensitivity, hepatic fat accumulation, and a reduction of lipogenic and inflammatory genes in the livers of mice on a Western diet for eight weeks. The combination of TRE with aerobic exercise provided complementary benefits regarding hepatic TG regulation and RER for males, while females exhibited better control of serum cholesterol, along with a reduction in the expression of the Fatp4 gene for both sexes. Aerobic exercise performance also improved for both, with males achieving superior results. Variations in metabolic responses between sexes were observed, highlighting the need for further studies. Additionally, the association of the Rev-erb? agonist with TRE brought improvements in glycemic homeostasis, insulin sensitivity, and serum cholesterol, contributing innovative data to the literature on this therapeutic combination (AU) | |
| FAPESP's process: | 21/13847-8 - Effects of time-restricted feeding combined with physical exercise or REV-ERB agonist on obesity and liver metabolism of male and female mice |
| Grantee: | Gabriel Calheiros Antunes |
| Support Opportunities: | Scholarships in Brazil - Master |