| Author(s): |
Adriana Franco Paes Leme
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2005-07-27 |
| Examining board members: |
Jaime Aparecido Cury;
Elaine Machado Benelli;
Jose Camillo Novello;
Sergio Roberto Peres Line;
Maria Regina Lorenzetti Simionato
|
| Advisor: | Jaime Aparecido Cury; Claudia de Mattos Bellato |
| Field of knowledge: | Health Sciences - Dentistry |
| Indexed in: | Base Acervus-UNICAMP |
| Location: | Universidade Estadual de Campinas. Faculdade de Odontologia de Piracicaba. Biblioteca Prof. Dr. Carlos Henrique Robertson Liberalli; T/UNICAMP; L542p |
| Abstract | |
Dental caries is a biofilm-dependent oral disease, and fermentable dietary carbohydrates are the key environmental factors involved with its initiation and development. Some hypotheses based on the structure, composition and ion kinetic aspects of biofilm have been suggested to explain the cariogenicity of biofilm formed in the presence of sucrose. Among them, the differential expression of bacteria proteins and the presence and absence of salivary proteins in biofilm formed in the presence and absence of sucrose has been suggested. Thus, this thesis was comprised by two manuscripts. The first discusses the role of sucrose in cariogenic dental biofilm formation and the second evaluates the proteins from biofilm formed in situ in the presence of sucrose. Among the hypotheses to explain the low inorganic concentration in the biofilm, the absence of calcium-binding proteins in biofilm formed in the presence of sucrose can help explain it. In the second study, calcium-binding proteins were identified only in biofilm formed in the absence of sucrose and help explain the higher calcium concentration in biofilm matrix. Proteins from oral microorganisms were also identified and most of them were associated to housekeeping functions, such as energy metabolism, amino acid biosynthesis, translation and stress-related proteins. Different stress-responsive proteins were expressed in the two conditions evaluated, suggesting specific adaptive-response in biofilms formed in the presence and absence of sucrose. Our results show that the characterization and the study of protein function in dental biofilm help explain important aspects involved with the initiation and development of dental carles (AU) | |