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| Author(s): |
Yara Maria Franco Moreno
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas |
| Defense date: | 2007-08-31 |
| Examining board members: |
Maria Marluce dos Santos Vilela;
Francisco Maugeri Filho;
Ronei Luciano Mamoni;
Maria Celia Cervi;
Adérson Omar Mourão Cintra Damião
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| Advisor: | Maria Marluce dos Santos Vilela; Marcos Tadeu Nolasco da Silva |
| Abstract | |
Special concerns have arisen in regard to HIV-1 infected children about their nutritional and health status due to the immunosuppressive effects. The development of strategies for improvement in the nutritional status and immune response is of great interest for immunocompromised patients. Nutritional supplementation with whey proteins is recognized as stimulating the immune system to increase glutathione synthesis. The levels of this antioxidant are reduced in HIV-1 infected individuals and are correlated with disease progression. With the objective of investigating the influence of oral supplementation with bovine whey proteins (native, hydrolyzed or enriched with prebiotics) on nutritional status, body composition, and innate and adaptative immune system in a group of HIV-1 infected children, between 2 and 16 years old, a prospective, double-blind clinical trial was conducted. During the experiment, children received oral whey proteins supplement for 3 months. The nutritional status was assessed by weight gain, growth and food intake while midarm circumference and skinfold thickness were used to determine body composition. The immune system was evaluated by the percentual of CD4+, CD8+ and gamma-delta T lymphocytes, BCG-specific lymphoproliferation and cytokine (TNF-a, IFN-?, IL-10 e IL-4) production on supernatant cultures and glutathione levels. Data were submitted to statistical analysis. The control group was composed of HIV-exposed but non-infected children. Oral supplementation did not influence nutritional status or body composition, but BCG-specific lymphoproliferation was enhanced in the group that received prebiotic enriched supplement. An increase of BCG-specific lymphoproliferation and IFN-? production was observed in the native whey protein supplemented group. These data suggest that nutritional supplementation with native or prebiotic enriched bovine whey proteins increase the BCG memory response in HIV-infected children (AU) | |