Study on the influence of radiation and pH at the knetic behavior of anthocynins from plants of hibiscus gender by chemometrics methods

Author(s)

Paulo Henrique Março

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Química
• Defense date: 2009-03-13

Examining board members

Ronei Jesus Poppi; Ieda Spacino Scarminio; Marco Flores Ferrão; Jarbas José Rodrigues Rohwedder; Adriana Vitorino Rossi

• Advisor: Ronei Jesus Poppi

Abstract

The influence of UV radiation and pH at the anthocyanin species equilibra, extracted from plants of Hibiscus gender was studied with chemometric methods. Anthocyanins from different extracts of 3 different flowers of Malvaceae family (Hibiscus Sabdariffa, Hibiscus Acetosella and Malvaviscus Penduliflorus) were used at this investigation. Spectrophotometric analysis at UV-Visible range were performed, comparing spectra and kinetic behavior from analysis with and without UV radiation exposure by using buffer solutions with pH varying from 1.5 to 12.7 and Flow Injection Analysis (FIA) to calculate the equilibra constants to the compounds found in the samples. Chemometric methods as Principal Components Analysis (PCA), Multy-Way PCA, Pure, Singular Value Decomposition (SVD), Multivariate Curve Resolution with Alterning Least Squares (MCR-ALS) were applied. Aiming to find out the number of species, as well as, to characterize them, the MCR-ALS method was applied by using individual and augmented matrix strategy. From the results of the chemometric methods application it was proposed an equilibra system to the species found at different pH values and from FIA results, the equilibra constants were calculated to some of the species (AU)

• FAPESP's process: 04/09231-6 - Study on the influence of radiation and pH at the kinetic behavior of anthocyanins from plants of Hibiscus gender by chemometric methods
• Grantee: Paulo Henrique Março
• Support Opportunities: Scholarships in Brazil - Doctorate