Evaluation in vitro of collagen type i production in MDPC-23 odontoblast-like cells and 3T3 fibroblasts after direct and indirect contact with bacteria involved in caries and endodontic infections

Author(s)

Claudia Leal Sampaio Suzuki

• Document type: Master's Dissertation
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2013-01-18

Examining board members

Alexandre Augusto Záia; Thais Accorsi Mendonça; Brenda Paula Figueiredo de Almeida Gomes

• Advisor: Alexandre Augusto Záia

Abstract

The aim of the study was to evaluate whether the bacteria Streptococcus mutans, Enterococcus faecalis and Porphyromonas gingivalis alter the secretion of type I collagen by 3T3 fibroblasts and MDPC-23 odontoblast-like cells in cell culture, and, with change, if it is dependent on the contact cell/bacteria or only bacterial by-products. 3T3 fibroblasts and MDPC-23 odontoblast-like cells were cultured and incubated with the bacteria S. mutans, E. faecalis and P. gingivalis (MOI 1:1) for the periods of 2, 4 and 8 hours. The bacteria were in direct contact with the cells, and indirect contact through the use of inserts 0.4 ?m. The production of type I collagen secreted by cells was quantified by Enzyme-Linked Immunosorbent Assay (ELISA), and its structural organization (birefringence) was visualized in polarized light microscopy after staining with picrosirus. Thus, it can be concluded that infection with S. mutans, E. faecalis and P. gingivalis stimulated an increase in the levels of type I collagen in periods 2 and 4 hours in cultured MDPC-23 odontoblast-like cells, and a progressive increase in the levels of type I collagen between 2 and 8 hours in cultured 3T3 fibroblasts. It was noted that the stimulus for the increase in collagen production is due to the action of the by-products released by bacteria and not by contact cell/bacteria (AU)

• FAPESP's process: 11/02719-7 - In vitro evaluation of the production of collagen type by odontoblasts and fibroblasts after direct and indirect contact with bacteria related to dental caries and endodontic infections
• Grantee: Claudia Leal Sampaio Suzuki
• Support Opportunities: Scholarships in Brazil - Master