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| Author(s): |
Vander Jose das Neves
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2012-02-24 |
| Examining board members: |
Fernanda Klein Marcondes;
Sergio Eduardo de Andrade Perez;
Carlos Alberto da Silva;
Claudio Alexandre Gobatto;
Maria Luiza Ozores Polacow
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| Advisor: | Fernanda Klein Marcondes |
| Abstract | |
The nandrolone or its association with physical training induced concentric cardiac hypertrophy associated with systolic and diastolic dysfunction in the heart of rat. In this way, to complement these results, the objective of this study was to evaluate the influence of nandrolone and resistance training on cardiac hypertrophy, the gene expression of pathological cardiac hypertrophy markers (ß-myosin heavy chain (ß-MHC), ß-myosin heavy chain (ß-MHC), ß-skeletal actin and atrial natriuretic peptide), blood pressure in vivo, cardiac electrophysiology, and the population of ß1-adrenoceptor (AR-ß1) and ß2 (ß2-AR) in right atrium of 88 Wistar rats, which were divided into four groups: non trained+vehicle (NTV), Trained+vehicle (TV), non trained+nandrolone (NTN), trained+nandrolone (TN). The animals were treated for 6 weeks with vehicle propylene glycol (0.2 mL/kg) or nandrolone decanoate (5 mg/kg), i.m. 2x/week. The training was carried out by jumping into water with overweight ranging from 50-70% of body weight, 5 days/week for 6 weeks. After sacrifice of animals, were performed the analysis of cardiac hypertrophy markers of ß adrenoceptors population. In other animals underwent the same treatment, blood pressure was analyzed 1x/semana and electrocardiogram was performed at the end of trial period. The results were calculated by two-way ANOVA followed by Tukey test and blood pressure by Analysis of Variance with Repeated Measures of Structure of Self-regressive Variance. The results showed cardiac hypertrophy 9% higher in the TV group and 8% for NTN compared to NTV, and this effect was potentiated in TN group. The nandrolone decreased the expression of ß-MHC in the NTN group (26%) and TN (42.5%), both compared to NTV. Training decreased the expression of ß-MHC in TV group (in 59.5%) and TN (22.7%) compared to NTV. Only in the TN group, both ß-skeletal actin (117.3%) and atrial natriuretic peptide (114%) were increased compared to NTV. In TV and TN groups, systolic, mean and diastolic blood pressures were decreased compared to the respective non trained groups. The heart rate decreased in the groups TV and TN in the weeks 5 and 6 in comparison to the respective groups NTV and NTN. The nandrolone increased systolic blood pressure in TN and NTN groups in the fifth week of treatment compared to the first and second weeks. The electrocardiogram analysis shown that nandrolone prolonged QTc interval by 3.7% in the NTN group and 2.7% in TN compared to respective groups NTV and TV. The nandrolone induced an increase of 50.1% in the population of AR-ß1 on NTN group and 75.6% in TN compared to NTV, and also increased 66.5% in the ß2-AR population in the NTN group and 126,7% in TN compared to NTV. In conclusion, the results of this study confirm that cardiac hypertrophy induced by only nandrolone or in combination with physical training is pathological, and suggest that changes in the population of ß-adrenoceptors are an attempt of right atrium to compensate the negative effects of nandrolone on the hypertrophied myocardium (AU) | |
| FAPESP's process: | 07/08462-2 - Analysis of cardiac hypertrophy induced by the o association of anabolic steroid and force training in rats. |
| Grantee: | Vander José das Neves |
| Support Opportunities: | Scholarships in Brazil - Doctorate |