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| Author(s): |
Juliana Lacorte Cazoto
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2012-03-12 |
| Examining board members: |
Marcelo Carnier Dornelas;
Paulo Mazzafera;
Juliana Lischka Sampaio Mayer;
Maria Helena de Souza Goldman;
Adriana Pinheiro Martinelli
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| Advisor: | Marcelo Carnier Dornelas |
| Abstract | |
During plant development, the shoot apical meristem (SAM) and the root apical meristem (RAM) are established during the embryo formation process, while the other organs as leaves, flowers and fruits are produced during the post-zygotic development. During plant embryogenesis, the expression of different transcription factors in a given time and place culminates with the activation of cellular domains responsible for the establishment and maintenance of the meristems. We hypothesized that the same genes that are activated during zygotic embryo development are also activated during somatic embryogenesis. Therefore we analyzed and compared the expression patterns of genes preferably expressed during embryo development in these two processes. For this purpose, we characterized anatomically both somatic and zygotic embryogenesis in Passiflora edulis (passion fruit). Later, we identified and characterized genes preferably expressed during somatic and zygotic embryogenesis in this species. Among the identified genes are members from YABBY, WOX and Aux/IAA families. These genes were selected by bioinformatics analysis from the PASSIOMA database of Passiflora reproductive expressed sequence tags (ESTs). The putative protein sequences encoded by the studied genes, together with possible horologes in Arabidopsis, were analyzed by sequence alignment. The expression patterns of these genes were studied by RT-PCR, qPCR and in situ hybridization during different developmental stages of P. edulis somatic and zygotic embryogenesis. The morpho-anatomical characterization of zygotic embryos in this specie resulted in a schedule elaboration that identifies, from the first day of pollination, the different developmental stages of the embryo. Moreover, the gene expression data suggest that the molecular mechanisms responsible for the processes of zygotic and somatic embryogenesis presented similar expression pattern during the beginning of the plant embryo development in P. edulis (AU) | |