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Analysis of bond strenght and hard tissues adjacent to restorative systems with or without fluoride release after cariogenic challenge in vitro

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Author(s):
Ana Paula Almeida Ayres
Total Authors: 1
Document type: Master's Dissertation
Press: Campinas, SP.
Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
Defense date:
Examining board members:
Marcelo Giannini; Patrícia Moreira Freitas; Flavio Henrique Baggio Aguiar
Advisor: Marcelo Giannini
Abstract

The aim of this study was to evaluate the bond strength to dentin of restorative systems containing or not fluoride in their compositions and the performance of enamel and dentin around the restorations, when submitted to cariogenic challenge in vitro. Additionally, the fluoride released was measured during the pH cycling. This study used 64 third molars human teeth and four restorative systems were selected: FL-Bond II/Beautifil II, Bond Force/Estelite Sigma, GC Fuji II LC and Adper Easy Bond/Filtek Z350 XT. For the bond strength evaluation, it was used oclusal dentin in which the restorative materials were applied (n=8). The "restored" teeth were sectioned for obtaining bonded beam specimens and the microtensile test was performed in two times: imediate and after one year of storage in artificial saliva. For the cross-sectional microhardness test, cervical cavities were prepared and filled with the restorative systems according to the manufacturer's instructions (n=8). The restored teeth were submitted to thermo cycling (3.000 cycles) and subsequent pH-cycling during eight days. The cross-sectional microhardness analysis was performed in enamel and dentin, at three distances from cavity wall and at six depths regarding the surface submitted to pH-cycling. The desmineralizing and remineralizing solutions of this pH-cycling regimen was used for fluor cationic concentration analysis. The statistical analysis of bond strength to dentin showed that means were not reduced after one year of storage in artificial saliva for no restorative systems. The results of cross-sectional microhardness measurements in enamel presented significant statistical difference only at the distance of 50 ?m. However, means of Knoop microhardness measurements were similar for the four restorative systems when compared to the same distances and depths. The same pattern of results was found in dentin, but the means of microhardness measurements showed no significant statistical differences between the distances from the restorations. Fluor cationic concentration analysis detected fluoride releasing by the specimens restored GC Fuji LC II and FL-Bond II/Beautifil II. The results of this in vitro study suggest that the restorative products tested presented bond stability after one year, but showed little ability to inhibit secondary caries around restoration, independently on fluoride releasing or not (AU)

FAPESP's process: 10/13601-4 - Evaluation of dentin-resin bond and the dentin adjacent to restorations with adhesive systems containing fluoride after in vitro cariogenic challenge.
Grantee:Ana Paula Almeida Ayres
Support Opportunities: Scholarships in Brazil - Master