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Evaluation of dentin-resin bond and the dentin adjacent to restorations with adhesive systems containing fluoride after in vitro cariogenic challenge.

Grant number: 10/13601-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2011
Effective date (End): February 28, 2013
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Marcelo Giannini
Grantee:Ana Paula Almeida Ayres
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


The adhesives and restorative materials have been modified to improve the quality of marginal sealing of restorations. However, the clinical performance of restorations with these materials, when evaluating the longevity and the development of caries around restoration is still unclear. This project aims to evaluate the restoration-dentin bonds and the behavior of the dentin adjacent to the restorations, which will be submitted to cariogenic challenge in vitro. This study will use human teeth and 4 restorative materials (glass ionomer resin modified, as the control group and three adhesives / composite resins). For the bond strength test, it will be used coronary dentin and the microtensile test will be conducted immediately after specimen preparation and 1 year after storage in artificial saliva. For the dentin longitudinal microhardness (or internal) and for polarized light microscopy analysis, the cavity preparation will be done in the cervical region (class V), which will be restored with the 4 restorative systems proposed. The teeth will be submitted to pH cycling for 8 days. Afterwards, they will be subjected to measurement of dentin microhardness (3 distances from the cavity wall and 6 depths from the surface that was treated by pH cycling) and evaluation of the depth and area of demineralization from the images obtained by polarized light microscopy.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALMEIDA AYRES, ANA PAULA; MACHADO TABCHOURY, CINTHIA PEREIRA; BERGER, SANDRINE BITTENCOURT; YAMAUTI, MONICA; BOVI AMBROSANO, GLAUCIA MARIA; GIANNINI, MARCELO. Effect of Fluoride-containing Restorative Materials on Dentin Adhesion and Demineralization of Hard Tissues Adjacent to Restorations. Journal of Adhesive Dentistry, v. 17, n. 4, p. 337-345, . (10/13601-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
AYRES, Ana Paula Almeida. Analysis of bond strenght and hard tissues adjacent to restorative systems with or without fluoride release after cariogenic challenge in vitro. 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Campinas, SP.

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