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Isolation, selection and study of microorganisms ligninolytic decomposers of manure

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Author(s):
Hamilton Roberto Fortes Bavutti
Total Authors: 1
Document type: Doctoral Thesis
Press: Campinas, SP.
Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos
Defense date:
Examining board members:
Lúcia Regina Durrant; Regina Monteiro; Tania Akiko Anazawa; Eglé Novaes Teixeira; Ranulfo Monte Alegre; Helia Harumi Sato; Fumio Yokoya
Advisor: Lúcia Regina Durrant
Abstract

The purpose of this work was to stablish parameters for the biological degradation of several leachate samples from a Campinas region's municipal sanitary landfill, to determine the kínd of microorganisms commonly found on these samples and to study their metabolism. Initally 323 strains were isolated using R2A medium. These strains were tested to toxical compounds resistance and ability to growth on lignin. Degradation of total leachate compounds, phenol degradation, DOO reduction and colour reduction were analysed in eíght different culture conditions. Four (4) fungal strains were more prepared to degrade the highly toxical leachate compounds. Strains 74, 100, 108 and 184 were tested and the strain 184 appeared to be the best in almost ali the conditions. One bacterial strain (254) also showed good results in some tests and deserves a detailed study. The maximum of DOO reduction was reached by the 184 strain in the experiments IV (51,8%) and V (50,4%). The best rates of phenol degradation were also reached by the strain 184 in the same experiments (72,9% and 63,7% respectiveUy). Strain 100 also presented good results and reduced the DOO around 36,8% in experiment 111and 38,6% in experiment IV. The same strain degradaded about 50,1% of phenol in the experiment IV. Non sterilized leachate added with glucose and yeast extract was better metabolized compared to non-added and sterilized leachate (AU)