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| Author(s): |
Hugo Takeda Caetano
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia |
| Defense date: | 2009-02-27 |
| Examining board members: |
Jose Camillo Novello;
Claudio Chrysostomo Werneck;
Carlos Alberto Labate
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| Advisor: | Jose Camillo Novello |
| Abstract | |
Along the last years, Proteomics has become an area of study each more essential to the better understanding of the effective and regulatory mechanisms of the cells and their interactions inside biological systems. Pathologies of genetic origin like the hypertriglyceridemia, that usually modify the lipid profile with the increase in the triglycerides and cholesterol levels, are strongly related to various cardiovascular diseases. Studies with animals and humans have been made in search of the understanding of 0011 and molecular mechanisms implied in these pathologies for potential therapeutic and preventive interventions. Studies in genetically hypertriglyceridemic mice to over expressing the human apolipoprotein C-III showed that high plasmatic concentrations of triglycerides and free fatty acids constitute a metabolic condition that changes the mitochondrial functionality. These changes in the breathing control of the transgenic mitochondria represent an adaptation attributed to structural or functional differences on the inner membrane of transgenic mitochondria. This way, we made the comparative study of the mitochondrial proteome obtained from these hypertriglyceridemic transgenic animals with the proteome from non transgenic control mice. For such, after the isolation of the normal and transgenic mitochondria, we used two-dimensional gel electrophoresis (2DE) and MALDI-ToF and Q-ToF mass spectrometry techniques to identify the differentially expressed proteins. Among them we identified the proteins Hydroxymethylglutaryl-CoA synthase, Carbamoyl-phosphate synthase and Electron transfer flavoprotein. These and other proteins can represent interesting molecular targets for the study of the mitochondrial functionality, aiming future clinical applications for the treatment of certain pathological conditions like hypertriglyceridemia. (AU) | |