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Morphological study of the vas deferens of domestic quail (Coturnix coturnix) of Italian variety: structure, histophysiology and possible seasonal variability along the year

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Author(s):
Katia Aparecida da Silva Viegas
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
Defense date:
Examining board members:
Antonio Marcos Orsi; Pedro Primo Bombonato; Joffre Guazzelli Filho
Advisor: Antonio Marcos Orsi
Abstract

The morphological, histomorphometric, ultrastructural and three-dimensional features of the vas deferens of quail (Coturnix coturnix) of the Italian variety were studied and described. For this study were utilized 30 adult sexually active quails taken from the experimental Edgardia Farm of the UNESP, Botucatu, São Paulo, Brazil. The vas deferens was collected, observed and documented along the year in the autumn, winter, spring and summer seasons, and studied by macro-mesoscopy, light microscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Each vas deferens was reduced in three segments: proximal (adepididymal); middle (just the median lobe of the kidney); and distal (adcloacal). The ultrastructural and three-dimensional studies were concentred in the middle segment of the duct. It was verified marked morphologic differences in the vas deferens, including structural and histomorphometric features concerning the middle values of the epithelial height, tubular and luminal diameters measured during the four seasons of the year. The observations made in the autumn the quiescent phase and in the spring the active phase of the annual testicular cycle were more evident. In the intermediaries phases of the cycle, i.e., the winter (recrudescent phase) and summer (regressive phase), the influence of the testicular kinetic on the vas deferens structure was noted mainly in the subcellular characteristics of principal cells of the epithelium lining. The SEM observations confirmed the morphologic variability of the vas deferens in the main seasons of the year (autumn and spring) concerning to the annual testicular cycle phases. These studies allowed to conclude that this avian species showed a circunannual pattern of the reproductive cycle, influencing the morphological features of the excurrent ducts of the testis (AU)