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| Author(s): |
Naianne Kelly Clebis
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2003-12-15 |
| Examining board members: |
Romeu Rodrigues de Souza;
Pedro Primo Bombonato;
Sandra Regina Stabille
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| Advisor: | Romeu Rodrigues de Souza |
| Abstract | |
The autonomous neuropathy present in animals with diabetes mellitus (DM) compromises several organic systems. On the gastrointestinal tract it may provoke diarrhea, constipation, estase and gastric dilation, leading to anorexia, weight loss and vomit. Although the triggering mechanisms of these disturbances are not completely clear, it seems that alterations of the myenteric plexus may be involved. The oxidizing stress - together with the sorbitol increase resulting from the metabolic alterations due to dm - results in an edema and in a neuronal lesion. Among the consequences of this lesion, alterations in the myenteric neurons have been described. The ascorbic acid (AA), besides its antioxidant effect, may also reduce the sorbitol concentration working as a neuroprotector. The aims of the present work were to investigate the possible alterations in the number and size of nadh-dr stained myenteric neurons of the stomach of diabetic rats and the effect of the aa-supplementation (1g/l of water). In order to do so, 15 rats (rattus norvegicus) were divided into three groups (n = 5): controls (C); diabetic (D); and diabetic supplemented with AA (DS). DM was induced by streptozotocin (35 mg/kg of body weight). 120 days later, the animals were sacrificed for obtaining their stomachs. The myentric neurons were stained employing the nadh-diaforase method. We counted the nadh-dr neurons employing the light microscope and we measured the cellular body profile (CBP) using an image analysis software. The aglandular and glandular areas of the stomach were analyzed separately. The number of neurons nadh-dr was larger (p <0,05) in the glandular area of group ds when compared to group d. The average of cbps was larger (p <0,05) for neurons of groups d and ds than for group c. There was an increase in the incidence of neurons with cbps over 200 mm2 in group D when compared to groups DS and C. The results suggest that the AA supplementation had a neuroprotetor effect on the nadh-dr myenteric neurons represented by the increase in the number of neurons and the decrease of big neurons in the glandular area of group DS. (AU) | |