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| Author(s): |
Matheus Levi Tajra Feitosa
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2008-08-29 |
| Examining board members: |
Carlos Eduardo Ambrósio;
Eduardo Harry Birgel Junior;
Flávia Thomaz Veréchia Pereira
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| Advisor: | Carlos Eduardo Ambrósio |
| Abstract | |
The osteonecrosis of femoral head (ONFH) is a degenerative disease that can lead to the collapse of the femoral head and necessity of total hip replacement. The main objective in the treatment of ONFH is to preserve the hip and not replace it. Studies in human patients with ONFH demonstrated that levels of activity and the number of mesenchymal cells in the hematopoietic and bone marrow stromal compartments are depressed. These findings indicate the possibility of the use of mesenchymal stem cells (MSC) in necrotic lesions on the head of the femur. This research proposes the use of cell therapy with MSC from ovine bone marrow and immature human dental pulp stem cell in ovine ONCF induced by intraosseous injection of ethanol, in addition, the assessment of bone restructuring by light microscopy and tracking of the transplanted cells. Two animals were euthanasied with 6 weeks after the induction of injury, and were transplanted mesenchymal stem cells in 4 animals, and held the central decompression in 2 other animals. After ten weeks were euthanasied the remaining animals. The successful of the bone marrow transplantation was confirmed by the macroscopic expression of the LacZ gene reporter in the femoral head of animals transplanted. The histological analysis showed an advanced regenerative process in the femoral head in the animal using human immature dental pulp stem cell. (AU) | |