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| Author(s): |
Katia Cristina Kimura
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2006-12-08 |
| Examining board members: |
Maria Lucia Zaidan Dagli;
José Luiz Guerra;
Paulo Hilario Nascimento Saldiva
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| Advisor: | Maria Lucia Zaidan Dagli |
| Abstract | |
The atmospheric pollution directly interferes with the population health. Evidences have been shown that pets represent sentinels for environmental factors which the man and the other animals would be susceptible. Animals can be used as indicatives of the environmental pollution. The industrial and urban developments have originated, in the whole world, an increase of the emission of atmospheric pollutants. The complex indoor "mixture of gases, solid particles and chemical substances in the air can induce genotoxicity, leading the citogenetic alterations, mutations or production of DNA adducts. Thus, this work has the objective to evaluate morforlogically the olfactory and pulmonary epitelia of 36 healthy dogs of the different regions of the city of São Paulo (zone East, West, North and South). The DNA breaks was analyzed by the Comet assay (Eletroforese in gel of isolated cell) and Micronucleus. The analysis of the 8´hydroxy 2´deoxyguanosine adduct was performed by Elisa test (Enzyme linked immunosorbent assay). The parameters were associated with the index of atmospheric pollution (PM10), supplied for the CETESB. The histopathological evaluation of the epithelium included the graduation of 0 to 3. No relationship with the index of atmospheric particulated material with the level of DNA breaks by the Comet assay in the olfactory epithelium was obtained. It does not have correlation between age and the injury of DNA for the Test of the Comet in the olfactory epithelium and neither for the pulmonary epithelium. It has significant correlation between the index of atmospheric particulate material and the injury of DNA in the pulmonary epithelium for the Comet assay. The use of the pulmonary epithelium in dogs in the COMET assay resulted significant, being able to be indicated as a bioindicador of atmospheric air. This project showed that the South region of the Sao Paulo city is apparently a less polluted region. The animals from that region show minor pulmonary alterations and lesser injury of DNA, in comparison with dogs from other regions. The micronucleus test did not show significant results. The Elisa test showed opposite results when compared to the Comet assay, suggesting its relation with the ozone index. (AU) | |