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| Author(s): |
Débora Dirani Sena de Gobbi
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2010-02-10 |
| Examining board members: |
Andrea Micke Moreno;
Simone de Carvalho Balian;
Terezinha Knöbl
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| Advisor: | Andrea Micke Moreno |
| Abstract | |
The species of Arcobacter spp. genus have been classified until 1990 as species belonging to the genus Campylobacter. Nowadays, three of five species of this genus are consider as potencially zoonotic, and can be transmitted from food of animal origin. The present study goal was to isolate and characterize genetically strains of Arcobacter spp. isolated from 120 carcasses, 120 faeces and 24 swine muscle sampled in two swine slaughterhouses located in São Paulo State. Isolates were submitted to multiplex-PCR to identify species of the genus and analyzed by pulsed field gel electrophoresis. The agent was isolated from 71,6% of carcasses, 4,16% of faeces and 8,3% of muscles samples. A. butzleri and A. cryaerophilus were the most prevalent species. The analysis of data obtained in PFGE showed 51 distinct profiles, the discriminatory index of 0,98 and it demonstrated large genotipic diversity among the strains. The main isolation site were the carcasses and the PFGE was a good tool to characterize and discriminate Arcobacter spp. strains. (AU) | |
| FAPESP's process: | 07/01636-5 - Isolation and characterization of Arcobacter spp. strains from intensive swine productions and slaughterhouses |
| Grantee: | Debora Dirani Sena de Gobbi |
| Support Opportunities: | Scholarships in Brazil - Master |