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Phylogenetic analysis and susceptibility antimicrobial profile of Arcobacter spp. strains isolated from poultry and porcine meat

Grant number: 14/07837-6
Support Opportunities:Regular Research Grants
Duration: June 01, 2014 - May 31, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal Investigator:Terezinha Knöbl
Grantee:Terezinha Knöbl
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Some species of Arcobacter are associated with food poisoning and may represent a biological hazard, associated frequently with poultry or porcine meat consume. In Brazil, this agent has been poor studied and there are few epidemiological studies in Southern region. The aim of this project is to characterize The antimicrobial profile of Arcobacter species, isolated from parts of chickens and porcine meat marketed in São Paulo, as well as perform a phylogenetic analysis of these isolates. A total of 200 cuts of breast, wing, thigh and drumstick of chicken and 200 cuts of prime rib, sirloin, steak and pork shank, coming from local markets, butchers and small markets in São Paulo will be assessed. After the step of pre-enrichment, samples are grown in a selective medium, Johnson and Murano. Bacterial species will be identified by molecular techniques (Polymerase chain reaction - PCR). The antimicrobial susceptibility profile will be determined by the minimum inhibitory concentration (MIC), Genotypic profile of the isolates will be evaluated by the AFLP technique (length polymorphism of amplified fragments). (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LILIAN APARECIDA SANCHES; MARCELO DA SILVA GOMES; RODRIGO HIDALGO FRICIELLO TEIXEIRA; MARCOS PAULO VIEIRA CUNHA; MARIA GABRIELA XAVIER DE OLIVEIRA; MÔNICA APARECIDA MIDOLLI VIEIRA; TÂNIA APARECIDA TARDELLI GOMES; TEREZINHA KNOBL. Captive wild birds as reservoirs of enteropathogenic E. coli (EPEC) and Shiga-toxin producing E. coli (STEC). Brazilian Journal of Microbiology, v. 48, n. 4, p. 760-763, . (14/07837-6)
XAVIER OLIVEIRA, MARIA GABRIELA; NOGUEIRA PRESSINOTTI, LEANDRO; SPINOLA CARVALHO, GIOVANE; VILELA OLIVEIRA, MIRELA CAROLINE; MORENO, LUISA ZANOLLI; CABRERA MATAJIRA, CARLOS EMILIO; SPINOLA BERGAMO, ALESSANDRO; MANUEL ALEIXO, VICTOR; CAIXETA VEIGA, ALEXANDRE; DE SOUZA CORSINO, ELVIS; et al. Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802). TROPICAL ANIMAL HEALTH AND PRODUCTION, v. 49, n. 4, p. 777-782, . (13/17136-2, 14/07837-6, 14/06584-7, 15/26159-1)
XAVIER DE OLIVEIRA, MARIA GABRIELA; DE MOURA GOMES, VASCO TULIO; VIEIRA CUNHA, MARCOS PAULO; MORENO, LUISA ZANOLLI; MORENO, ANDREA MICKE; KNOBL, TEREZINHA. Genotypic Characterization of Arcobacter spp. Isolated from Chicken Meat in Brazil. FOODBORNE PATHOGENS AND DISEASE, v. 15, n. 5, p. 293-299, . (14/07837-6, 14/06584-7)
SANCHES, LILIAN APARECIDA; GOMES, MARCELO DA SILVA; FRICIELLO TEIXEIRA, RODRIGO HIDALGO; VIEIRA CUNHA, MARCOS PAULO; XAVIER DE OLIVEIRA, MARIA GABRIELA; MIDOLLI VIEIRA, MONICA APARECIDA; TARDELLI GOMES, TANIA APARECIDA; KNOBL, TEREZINHA. Captive wild birds as reservoirs of enteropathogenic E-coli (EPEC) and Shiga-toxin producing E-coli (STEC). Brazilian Journal of Microbiology, v. 48, n. 4, p. 760-763, . (14/07837-6)
GUERRA, MARIA F. L.; TEIXEIRA, RODRIGO H. F.; RIBEIRO, VANESSA L.; CUNHA, MARCOS P. V.; OLIVEIRA, MARIA G. X.; DAVIES, YAME M.; SILVA, KETRIN C.; SILVA, ANA P. S.; LINCOPAN, NILTON; MORENO, ANDREA M.; et al. Suppurative peritonitis by Klebsiella pneumoniae in captive gold-handed tamarin (Saguinus midas midas). JOURNAL OF MEDICAL PRIMATOLOGY, v. 45, n. 1, p. 42-46, . (14/07837-6)

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