Arcobacter spp. in fecal samples from Brazilian farmed caimans (Caiman yacare, Daudin 1802)

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Xavier Oliveira, Maria Gabriela ; Nogueira Pressinotti, Leandro ; Spinola Carvalho, Giovane ; Vilela Oliveira, Mirela Caroline ; Moreno, Luisa Zanolli ; Cabrera Matajira, Carlos Emilio ; Spinola Bergamo, Alessandro ; Manuel Aleixo, Victor ; Caixeta Veiga, Alexandre ; de Souza Corsino, Elvis ; Guarnieri Christ, Ana Paula ; Zanolli Sato, Maria Ines ; Moreno, Andrea Micke ; Knobl, Terezinha

• Document type: Journal article
• Source: TROPICAL ANIMAL HEALTH AND PRODUCTION; v. 49, n. 4, p. 777-782, APR 2017.
• Web of Science Citations: 0

Abstract

The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans. (AU)

• FAPESP's process: 13/17136-2 - Characterization of Leptospira strains isolated in Brazil by pulsed field gel electrophoresis (PFGE) typing and comparative genomic analysis.
• Grantee: Luisa Zanolli Moreno
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/07837-6 - Phylogenetic analysis and susceptibility antimicrobial profile of Arcobacter spp. strains isolated from poultry and porcine meat
• Grantee: Terezinha Knöbl
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/06584-7 - Genotypic characterization of Arcobacter spp. isolated from poultry meat marketed in São Paulo-SP
• Grantee: Maria Gabriela Xavier de Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/26159-1 - Comparative genome analysis of Streptococcus suis strains isolated from swine in Brazil
• Grantee: Carlos Emilio Cabrera Matajira
• Support Opportunities: Scholarships in Brazil - Doctorate