Total and differential cellular count in milk and blood from healthy goats bred in Sao Paulo state

Author(s)

Karina Medici Madureira

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD)
• Defense date: 2006-01-30

Examining board members

Wanderley Pereira de Araujo; Alice Maria Melville Paiva Della Libera; Angélica Trazzi Bento de Moraes

• Advisor: Wanderley Pereira de Araujo

Abstract

The present investigation had the objective to evaluate quantitatively and qualitatively the celularity of created healthy goats in Sao Paulo state, as well as correlating the somatic cells of milk with the leukocytes in the blood, information that, beyond rare in relation to the mammary gland of healthy goats, are extremely conflicting. Samples of each mammary half had been harvested, proceeding from 51 Saanen, Alpina and Toggenburg healthy goats, in different phases of lactation, from the different breeders of Sao Paulo state. The milk samples had been submitted to California Mastitis Test (CMT), the electronic counting of somatic cells and direct microscopy, using the pyronin Y-methyl green stain, beyond the cytospin centrifuge for the evaluation of leukocytes; with the samples of blood the leucogram was carried through. Associating you prop up of the CMT with electronic the cellular counting had been found the medium values of 181.000, 578.000, 628.000, 1.421.500 and 5.542.000, respectively. Already in the cellular counting for direct microscopy the gotten medium values had been of 74.991, 271,396, 71,420, 640,995 and 5.049.394, corresponding, respectively to negative, traces, 1+, 2+ and 3+ scores. Becoming related the cellular counting in accordance with the phases of the lactation; the gotten medium values had not presented significant differences between the phases, initial, intermediate and final of the lactation in such for the electronic counting how much for the direct microscopically counting, respectively. The correlation between the automatic and direct microscopically counting cellular was of 88%. Significant differences had not been found when the counting of somatic cells in the glands was compared right and left, as much in the microscopically automatic counting in the direct one. The relative values of the leucocytes gifts in milk had been determined, getting the average values and 77,0 ± 30,5%; 76,5 ± 20.9% and 65,3 ± 25.6% of neutrophils, 2,24 ± 3,26%; 2,04 ± 2.33% and 2,14 ± 2.81% of lymphocytes and 22,6 ± 20.2%, 21,4 ± 20.0% and 23,0 ± 16.2% of monocytes, corresponding the phases initial, intermediate and final of the lactation, respectively. In the blood, the total value of leucocytes was determined, getting itself the average value of 8525 ± 3244 céls/mL. The average relative values of the different types of leukocytes gifts in the blood of healthy goats had been of 56,8 ± 11.8% of neutrophils, 38,5 ± 11.8% of lymphocytes, 2,8 ± 1.7% of monocytes and 1,9 ± 1.6% of eosinophils. Correlating the leukocytes gifts in the blood with the gifts in milk, significant correlation had not been observed, however, when it analyzed the leucocytes of the blood with the phases of the lactation, significant differences in the average relative values of neutrophils and lymphocytes had been found, respectively, in the final phase of lactation. The gotten results allow to conclude that difference between the automatic cellular counting and the direct microscopically one in the determination of the somatic frame number in goat milk exists, the cellular values increase as prop up it of the CMT, the predominant leucocyte in milk and blood of healthy goats is neutrophil, exists close relation between neutrophil and lymphocytes in the blood of goats, being these influenced by the phase of the lactation. (AU)