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| Author(s): |
David Marques de Almeida Spadotti
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2012-11-23 |
| Examining board members: |
Jorge Alberto Marques Rezende;
Kátia Regiane Brunelli;
Renate Krause Sakate
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| Advisor: | Jorge Alberto Marques Rezende |
| Abstract | |
Zucchini yellow mosaic virus (ZYMV) is probably one of the most dynamics and economically important emerging plant viruses. Since it was first report in Italy and France in 1973 and 1979, respectively, ZYMV has been found in over than 50 countries ranging from tropical to temperate climate conditions in all continents, except in Antartida. In Brazil this potyvirus was first reported in the beginning of 90´s in São Paulo and Santa Catarina States, and then in Ceará, Bahia, Rio Grande do Norte, Pará, Mato Grosso do Sul and Maranhão states. Probably it occurs in all Brazilian states which grow cucurbit species. Although ZYMV occurs in Brazil for more than 20 years, there is little information about the biological, serological and molecular characteristics for the isolates found in the country. The purpose of this work was to cover partially this gap to provide subsidies for genetic breeding programs. Eleven isolates collected in several Brazilian states were used. The biological characterization consisted in the study of the reaction of several vegetal species mechanically inoculated with the virus. The serological characterization consisted in the identification of the molecular weight of the coat protein through western blot analysis. The molecular characterization was done by the analyses of nucleotides and deduced amino acids sequences for the coat protein gene. The host reaction showed slight variation according to the virus. RFLP analyses showed that restriction enzyme HpaII allowed differentiate the mild ZYMV-M isolate from the majority of severe isolates. The coat protein molecular weight for all studied isolates was about 36 KDa, typical of the coat protein of this potyvirus. The nucleotide identity of the coat protein gene among the ZYMV Brazilian isolates ranged from 93% to 100%, and the deduced amino acids sequences ranged from 97% to 100%. Compared to corresponding sequences of ZYMV isolates from different geographical locations the nucleotide sequences identity ranged from 82% to 99%, while the deduced amino acids sequences ranged from 87% to 99%. Phylogenetic analysis place the Brazilian isolates of ZYMV into the group A, subdivision I or II, showing some diversity between the isolates. No recombination event was detected in the Brazilian isolates. (AU) | |
| FAPESP's process: | 10/13679-3 - Biological, serological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus |
| Grantee: | David Marques de Almeida Spadotti |
| Support Opportunities: | Scholarships in Brazil - Master |