Full text
| |
| Author(s): |
Fábio Pértille
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2013-02-06 |
| Examining board members: |
Luiz Lehmann Coutinho;
Gerson Barreto Mourão
|
| Advisor: | Luiz Lehmann Coutinho |
| Abstract | |
Within the livestock sector, the broiler industry has showed fastest growing rates in past decades. Those advances were achieved mainly because a better understanding of the nutrition requirements, animal management and animal genetics. Biotechnology has gained a prominent role with the use of molecular markers as a tool for adding genomic information to conventional breeding processes. Previous studies using an F2 population developed from a broiler x layer cross led to the identification of a SNP on the Fibroblast growth factor binding protein 1 (FGFBP1) (g. 2014G> A) on Gallus gallus chromosome 4 (GGA4). This gene is part of a QTL associated with thigh & drumstick yields, weight gain at 35 and 41 days. This paper investigates the previously identified QTL for the identification of additional polymorphisms and their associations with important economic traits using a single and multiple markers tests. Three positional candidate genes were identified on the QTL region: KLF3 (Krüeppel-like factor 3), SLIT2 (Slit homolog 2) and PPARG (Peroxisome proliferator-activated receptor gamma, coactivator 1alpha). Sequencing of those genes was conducted in eleven (n=11) F1 animals and one polymorphism was identified in each gene g.6763 C> T (KLF3), g.187737 C> A (SLIT2) and g.76638826 -/TTTCT (PPARGC1A). These mutations were genotyped in an F2 (n=276) and a pure broiler line (n=840) from Embrapa. The frequency of the genes alleles were: KLF3 gene in F2 population C = 50% T = 50% and pure TT population C = 98% T = 2%; SLIT2 gene in F2 population A = 25% C = 75% and pure TT population A = 30% C = 70%; PPARGC1A gene in F2 population Del =43% and In = 57% and pure TT population Del = 33% C = 67% indicating that those polymorphisms are still segregating in both populations. Association was identified (P < 0,05) with several carcass and development traits in the F2 and Pure TT population, some which were confirmed like liver, drumstick weights, weight gain from 35 to 41 days with g.6763 C> T (KLF3) and wings, had, carcass, back, drumstick, breast and liver and abdominal fat weights with g.76638826 -/TTTCT (PPARGC1A) indicating that this QTL region is important for production and performance traits of broiler. (AU) | |
| FAPESP's process: | 11/05319-0 - Identification of polymorphisms associated with performance and carcass traits in chromosome 4 of chicken |
| Grantee: | Fábio Pértille |
| Support Opportunities: | Scholarships in Brazil - Master |