Full text
| |
| Author(s): |
Carla de Andrade
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2013-03-04 |
| Examining board members: |
Valdomiro Shigueru Miyada;
Dirlei Antonio Berto;
Jose Eurico Possebon Cyrino;
José Fernando Machado Menten;
Aline Mondini Calil Racanicci
|
| Advisor: | Valdomiro Shigueru Miyada |
| Abstract | |
The purpose of this study was to evaluate the effects of dietary nucleotide levels on performance, occurrence of diarrhea, organ morphometry, intestinal histology and intestinal microbiota of weanling pigs fed complex diets containing corn, soybean meal, milk products, and spray-dried plasma. One hundred and sixty 21d-weaned pigs, averaging 6.43 ± 0.71 kg BW, were used in a randomized complete block design experiment with 5 treatments, 8 replications (blocks) per treatment and 4 animals per experimental unit (pen). The treatments were: basal diet with 120 ppm of chloro-hydroxyquinoline (antimicrobial treatment), and basal diet with 0, 100, 150, and 200 ppm of nucleotides. The ADG, ADFI, G:F and occurrence of diarrhea were calculated during 1 to 14 and 1 to 34 d of experimental period. A day after the end of the experimental period, an animal from each pen was slaughtered to evaluate of organ morphometry (empty stomach, empty small intestine, pancreas, liver and spleen), intestinal histology (villus height, villus width, crypt depth and villus height-to-crypt depth ratio), and intestinal microbiota (mesophiles, Gram-positive bacteria, Gram-negative bacteria, Lactobacillus spp., Escherichia coli, Salmonella spp., Staphylococcus spp., and Clostridium perfringens). During 1-14 d of experimental period, performance was not affected (P>0.05) by treatments. For the total experimental period (1-34 d), beneficial linear effects of dietary levels of nucleotides on final BW (P=0.005; BW = 0.0033X + 23.657, R2 = 0.87) and ADG (P = 0.008; ADG = 0.0002X + 0.4955, R2 = 0.83) were observed, but not (P>0.05) on ADFI and G:F. Pigs fed antimicrobial treatment had lower (P=0.049) occurrence of diarrhea from d 1 to 14 than those fed nucleotide treatments. However, for the total experimental period (1-34 d), treatments did not affect (P>0.05) occurrence of diarrhea, organ morphometry, intestinal histology and intestinal microbiota. Therefore, adding up to 200 ppm of dietary nucleotides to complex diets for weanling pigs showed beneficial effect on growth performance, without affecting organ morphometry, intestinal histology, intestinal microbiota and occurrence of diarrhea of nursery pigs. (AU) | |
| FAPESP's process: | 10/07318-8 - Nucleotides in feed for weanling pigs |
| Grantee: | Carla de Andrade |
| Support Opportunities: | Scholarships in Brazil - Doctorate |