Full text
| |
| Author(s): |
Flávia Regina Capaldi
Total Authors: 1
|
| Document type: | Master's Dissertation |
| Press: | Piracicaba. |
| Institution: | Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) |
| Defense date: | 2002-04-05 |
| Examining board members: |
Antonio Natal Gonçalves;
Luiz Humberto Gomes;
Adriana Pinheiro Martinelli Rodriguez
|
| Advisor: | Antonio Natal Gonçalves |
| Abstract | |
Cryptomeria japonica D. Don. "elegans" is a fast-growing tree belonging to the Taxodiaceae and it is considered a responsive species 'in vitro'. The aim of this work was to evaluate the effect of ten different concentrations of nitrate and ammonium in morphogenesis 'in vitro' of Cryptomeria japonica D. Don. "elegans" explants. Analysis of relative growth rate, total soluble proteins, total soluble aminoacids, total non structural carbohydrates and electrophoresis of total proteins was performed in order to observe the role of different nitrogen sources on the growth and development of the explants cultivated 'in vitro'. The concentratios of nitrate and ammonium that showed the best results were selected and used in an experiment with different concentrations of NAA and BAP to observe the shoot production. Each experiment was analised at the 30 th , 60 th and 90 th days of culture. The biochemical analysis was performed only at 90 th day of cultive. Concentrations from 26 to 31mmol.L -1 of NO3 - and lower than 5mmol.L -1 of NH4 + were the most effective for growth and development of the explants. However, the experiment with different concentrations of NNA and BAP showed that the best shoot production was achieved on 22,5mmol.L -1 of NO3- + 2,5mmol.L -1 of NH4 + and on 25,0mmol.L -1 of NO3 - + 5,0mmol.L -1 of NH4 + and with 2,0mg.L -1 of BAP + 1,0mg.L -1 of NNA. (AU) | |