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| Author(s): |
Mariana Ferreira Leite
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) |
| Defense date: | 2006-07-20 |
| Examining board members: |
Jose Nicolau;
Jarbas Arruda Bauer;
Antonio Carlos Lopes;
Fernando Neves Nogueira;
Marinilce Fagundes dos Santos
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| Advisor: | Jose Nicolau |
| Abstract | |
The aim of study was evaluate the effect of sodium tungstate administration (2mg/ml) on some parameters of parotid, submandibular and pilocarpine/isoproterenol stimulated saliva of streptozotocin induced-diabetes, during six weeks. The groups were divided in untreated control (C), treated control (CT), untreated diabetic (D) and treated diabetic (DT). The parameters studied were energetic metabolism, proteic composition of glands and saliva, besides the protein secretion pathway. In the energetic metabolism were determinated hexokinase, phosphofructokinase-1, pyruvate kinase, glycose-6-phosphate dehydrogenase and lactate dehydrogenase activities. Were evaluated the total protein concentration, amylase and peroxidase activities and free and total sialic acid content on saliva and salivary glands. The protein secretion pathway was studied by evaluation of expression of protein kinase C by western blot. The results obtained confirm the tungstate potential as hypoglycemic, as well as its action in the polifagia, polidipsia and body and glandular weight control. The glycogen concentration suffered an increment in the diabetic group and the tungstate treatment potentialized the increase in the salivary glands. Parotid glands suffered an increased in some parameters of glycolitic pathway and its protein composition in the initial weeks of study, with normalization in the end of experiment. The tungstate was effective in the control of peroxidase activity in salivary glands, but few effects on the other parameters. Diabetic animals presented an increased of total protein concentration in saliva, but no difference was observed in the amylase and peroxidase activities. Sodium tungstate caused an increased in the total protein concentration and a reduction on amylase activity of saliva in the CT and DT groups. Submandibular gland of diabetic rats suffered stimulation in the active and inactive PKC expression after one week and alteration in the isoforms profile of enzyme. Sodium tungstate potencialized this increased. Conclusion: In this study, was not observed effect of sodium tungstate on energetic metabolism of parotid and submandibular, showing that this compound does not alter the metabolism in the peripheral tissue as salivary glands. The tungstate acts on peroxidase activity, this show the possible action of this compound in the antioxidant system. Sodium tungstate stimulates the protein secretion pathway in the salivary glands. (AU) | |