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| Author(s): |
Luciana Fasanella Matizonkas Antonio
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) |
| Defense date: | 2006-02-03 |
| Examining board members: |
Fabio Daumas Nunes;
Andrea Mantesso;
Pedro Michaluart Junior;
Luciana Maria Pedreira Ramalho;
Renata Tucci
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| Advisor: | Fabio Daumas Nunes |
| Abstract | |
Homeobox genes comprise a family of developmental regulators that are vital for several aspects of growth and differentiation. Recently HOXA7, HOXC6 and TGIF genes have been implicated with carcinogenesis and tumoral progression. However their involvement with oral squamous cell carcinomas (OSCC) has not been demonstrated yet. The possible presence of HOXA7, HOXC6 and TGIF transcripts in OSCC and adjacent non-tumoral tissues (NT) was verified. Transcripts were amplified by RT -PCR and its cellular localization was determined by in situ hybridization with specific riboprobes. Amplification of HOXA7 was seen in 70% of cases, 15% only in NT tissues, 45% only in OSCC samples and 10% in both tissues. No amplification of HOXC6 was observed. TGIF was amplified in 80% of cases, 5% only in NT tissues, 20% only in OSCC samples and 55% in both tissues. Statistical analysis showed that there was no significant difference between amplification of HOXA7 or TGIF and the type of tissue analyzed. Moreover, no association between amplification of transcripts in OSSC and clinical aspects was observed. ISH signal was similar for HOXA7 and TGIF transcripts. In NT tissues there was an intense expression in the epithelium, either in basal and suprabasal layers or disperse and more intense in the spinous layer. In OSCC transcripts were locali zed in all tumoral cells, but in poorly differentiated areas the signal was less intense. These results show that HOXC6 was not involved in oral carcinogenesis while the presence of HOXA7 and TGIF transcripts in OSSC, mostly in well differentiated regions, suggests a participation of these genes in OSCC (AU) | |