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Identification of molecular profiles associated to the development of oral squamous cell carcinoma using the cDNA microarrays technology

Grant number: 10/08400-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2010
Effective date (End): August 31, 2014
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal researcher:Alfredo Ribeiro da Silva
Grantee:João Paulo Oliveira da Costa
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Oral squamous cell carcinoma (OSCC) is the most common cancer in oral cavity, corresponding more than 95% of oral tumors, and have been implicated in worst prognosis and high morbity when diagnosed in advanced stages. Therefore, a little is known about changes in genetic expression during the development of OSCC. In this study, using cDNA microarrays, our aim is to verify differences in genetic expression profiles between tumors in different stages and relate this expression patterns to clinicopathological and microscopical findings in OSCC. From A.C. Camargo Cancer Hospital's Biobank will be retrieved ten frozen samples from each clinical stage (I, II, III and IV), and ten normal mucosa samples. Using cDNA microarrays technique, we will evaluate the whole human genome expression in each tumor, and compare the expression between each stage and normal tissues. Differentially expressed genes will be identified using the statistical test for microarray analysis SAM (Significance Analysis of Microarrays). To confirm the results from cDNA microarray assay, qRT-PCR will be performed, in order to confirm overexpression seen in cDNA microarray and compared with clinicopathological and microscopical findings. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA-COSTA, JOAO PAULO; DE CARVALHO, ALEX FIORINI; DA SILVEIRA, GIORGIA GOBBI; AMAYA, PETER; WU, YONGQI; PARK, KYOUNG-JOO JENNY; GIGLIOLA, MABEL PINILLA; LUSTBERG, MARYAM; CAVICCHIOLI BUIM, MARCILEI ELIZA; FERREIRA, ELISA NAPOLITANO; KOWALSKI, LUIZ PAULO; CHALMERS, JEFFREY J.; SOARES, FERNANDO AUGUSTO; CARRARO, DIRCE MARIA; RIBEIRO-SILVA, ALFREDO. Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells. ONCOTARGET, v. 6, n. 25, p. 20902-20920, AUG 28 2015. Web of Science Citations: 40.
OLIVEIRA-COSTA, JOAO PAULO; OLIVEIRA, LUCINEI ROBERTO; DA SILVEIRA, GIORGIA GOBBI; SOAVE, DANILO FIGUEIREDO; SOARES, FERNANDO AUGUSTO; RIBEIRO-SILVA, ALFREDO. Topoisomerase expression in oral squamous cell carcinoma: relationship with cancer stem cells profiles and lymph node metastasis. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 41, n. 10, p. 762-768, NOV 2012. Web of Science Citations: 6.
OLIVEIRA-COSTA, JOAO PAULO; ZANETTI, JULIANA S.; SILVEIRA, GIORGIA G.; SOAVE, DANILO F.; OLIVEIRA, LUCINEI R.; ZORGETTO, VERONICA A.; SOARES, FERNANDO A.; ZUCOLOTO, SERGIO; RIBEIRO-SILVA, ALFREDO. Differential expression of HIF-1 alpha in CD44(+)CD24(-/) (low) breast ductal carcinomas. DIAGNOSTIC PATHOLOGY, v. 6, AUG 8 2011. Web of Science Citations: 16.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.