Rheological and enthalpy performance of pan bread dough with maize resistant starch and transglutaminase.

Author(s)

Diana Buchner de Oliveira Sanchez

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Escola Politécnica (EP/BC)
• Defense date: 2009-05-15

Examining board members

Carmen Cecília Tadini; Janaina Aparecida Mainardi; Maria Cecilia Puppo

• Advisor: Carmen Cecília Tadini

Abstract

The objective of this work was to study the partial substitution of wheat flour (WF) to maize resistant starch (MRS) and the addition of transglutaminase enzyme (TG) on the rheological and enthalpy properties of pan bread dough. The MRS was added as an alternative to increase the fiber ingestion while TG was added to supply the gluten dilution, catalyzing the bonds. Texture analyses (uniaxial extension, biaxial extension and Texture Profile Analysis TPA), thermal analysis on Differential Scanning Calorimetric (DSC) and rheological tests (rheofermentometer) were carried out in pan bread dough produced with five levels of wheat flour substitution from maize resistant starch: 1.5 %; 3.5 %; 8.5 %; 13.5 %; 15.5 % (basis on 100 % of mixture) and five addition levels of transglutaminase (TG): 0.03 %; 0.05 %; 0.10 %; 0.15 %; 0.17 % according to a composite design (2²) with three central and four star points. The same tests were conducted in pan bread dough without added MRS and TG and considered as control. The substitution of WF from MRS has more influenced the textural parameters than the addition of TG. The extension strength achieved a value 32 % higher than control with the substitution of 8.5 % of MRS and 0.12 % of TG. During the DSC tests, it has been found three endothermic peaks: fusion of water, gelatinization of wheat starch (60 °C) and gelatinization of MRS (115 °C). The maximum dough height (Hm), obtained from rheofermentometer curves, was significantly influenced, decreasing with the increase the addition of MRS. The absorption of water increased with the fibers addition, however up to 11 % of substitution of MRS, it could be considered equal to the control. TG did not contribute positively on the dough performance, at studied interval, and therefore mixture with substitution of WF by MRS up to 8.5 % will not significantly influence their rheological and texture parameters and can be used to manufacture pan bread with high fiber content. (AU)