Regeneração da medula espinhal de ratos adultos após a inoculação de células de Schwann em presença do fator neurotrófico derivado de células Gliais (GDNF). Análise comportamental e celular.

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Author(s):	Ricardo José de Almeida Leme Total Authors: 1
Document type:	Doctoral Thesis
Press:	São Paulo.
Institution:	Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:	2004-06-09
Examining board members:	Gerson Chadi; Dania Emi Hamassaki; Andre Fabio Kohn; Francesco Langone; Maria da Graça Naffah Mazzacoratti
Advisor:	Gerson Chadi
Abstract
Schwann cells (SC) obtained from rat sciatic nerve were grafted, in the presence or not of glial derived neurotrophic factor (GDNF), in adult rats submitted to a complete spinal cord transection at T11. Behavioral analysis from the 1st to the 3rd post operative month and of the immunoreactive fiber outgrowth in the graft epicenter at the end of this period showed: temporal improvements on motor parameters that were superior in the groups that received SC, being even higher in the presence of GDNF; increased amount of axonal fibers in the groups treated with SC, being even higher in the presence of GDNF. In another experiment, astrocytic and microglial activation and astrocytic neurotrophic factor bFGF expression were analyzed after a partial spinal cord transection, 1 week and 3 months after surgery. Astrocitic and microglial activation as well as a higher intensity of astrocitic bFGF in the white and gray matters were found in the entire spinal cord, what is related to the local scar formation and distant trophic and plastic phenomena. (AU)