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| Author(s): |
Pedro Omori Ribeiro de Mendonça
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-11-26 |
| Examining board members: |
Claudimara Ferini Pacicco Lotfi;
Madson Queiroz de Almeida;
Sonir Roberto Rauber Antonini;
Maria Candida Barisson Villares Fragoso;
Patricia Gama
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| Advisor: | Claudimara Ferini Pacicco Lotfi |
| Abstract | |
The proliferation of adrenal cortical cells involves genes and proteins related with the control of the cell cycle. The main factor involved in the proliferation of the adrenal cortex is the ACTH (corticotrophin), but other factor, such as the peptide N-terminal of POMC, seems to be involved. To contribute to the understanding of the mechanisms involved in the proliferative response of the adrenal cortex, we analyzed the effects triggered by both peptides in rats with the HPA axis inhibited by dexamethasone. The methods used were the incorporation of BrdU; analysis of protein cyclins D, E and p27 expression through immunohistochemistry and immunoblotting and expression of genes involved in cell cycle regulation by using qRT-PCR arrays. The results suggest that both peptides induced proliferative effect in adrenal cortex in a zone-specific manner. This effect may be mediated by the control of cyclins D2, D3 and E expression, and also by the cell cycle inhibitor, protein p27KIP1. (AU) | |
| FAPESP's process: | 08/00346-6 - In vivo effects of ACTH and N-POMC peptide on gene expression related with proliferation and celular death of rat adrenal |
| Grantee: | Pedro Omori Ribeiro de Mendonça |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |